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dc.contributor.authorEstaleva, Calvina E.L.
dc.contributor.authorZimba, Tomas F.
dc.contributor.authorSekyere, John Osei
dc.contributor.authorGovinden, Usha
dc.contributor.authorChenia, Hafizah Y.
dc.contributor.authorSimonsen, Gunnar S.
dc.contributor.authorHaldorsen, Bjørg
dc.contributor.authorEssack, Sabiha Y.
dc.contributor.authorSundsfjord, Arnfinn
dc.date.accessioned2021-07-07T10:47:00Z
dc.date.available2021-07-07T10:47:00Z
dc.date.issued2021-01-06
dc.description.abstractBackground: Epidemiological data of cephalosporin-resistant Enterobacterales in Sub-Saharan Africa is still restricted,and in particular in Mozambique. The aim of this study was to detect and characterize extended-spectrum β-lactamase (ESBL) - and plasmid-mediated AmpC (pAmpC)-producing clinical strains of Escherichia coli at Maputo Central Hospital (MCH), a 1000-bed reference hospital in Maputo, Mozambique. Methods: A total of 230 clinical isolates of E. coli from urine (n = 199) and blood cultures (n = 31) were collected at MCH during August–November 2015. Antimicrobial susceptibility testing was performed by the disc diffusion method and interpreted according to EUCAST guidelines. Isolates with reduced susceptibility to 3rd generation cephalosporins were examined further; phenotypically for an ESBL−/AmpC-phenotype by combined disc methods and genetically for ESBL- and pAmpC-encoding genes by PCR and partial amplicon sequencing as well as genetic relatedness by ERIC-PCR. Results: A total of 75 isolates with reduced susceptibility to cefotaxime and/or ceftazidime (n = 75) from urine (n = 58/199; 29%) and blood (n = 17/31; 55%) were detected. All 75 isolates were phenotypically ESBL-positive and 25/75 (33%) of those also expressed an AmpC-phenotype. ESBL-PCR and amplicon sequencing revealed a majority of blaCTX-M (n = 58/75; 77%) dominated by blaCTX-M-15. All AmpC-phenotype positive isolates (n = 25/75; 33%) scored positive for one or more pAmpC-genes dominated by blaMOX/FOX. Multidrug resistance (resistance ≥ three antibiotic classes) was observed in all the 75 ESBL-positive isolates dominated by resistance to trimethoprimsulfamethoxazole, ciprofloxacin and gentamicin. ERIC-PCR revealed genetic diversity among strains with minor clusters indicating intra-hospital spread. Conclusion: We have observed a high prevalence of MDR pAmpC- and/or ESBL-producing clinical E. coli isolates with FOX/MOX and CTX-Ms as the major β-lactamase types, respectively. ERIC-PCR analyses revealed genetic diversity and some clusters indicating within-hospital spread. The overall findings strongly support the urgent need for accurate and rapid diagnostic services to guide antibiotic treatment and improved infection control measures.en_US
dc.identifier.citationEstalva, Zimba, John, Simonsen GS, Haldorsen BC, Essack S, Sundsfjord A. High prevalence of multidrug resistant ESBL- and plasmid mediated AmpC-producing clinical isolates of Escherichia coli at Maputo Central Hospital, Mozambique. BMC Infectious Diseases. 2021;21(18)en_US
dc.identifier.cristinIDFRIDAID 1873565
dc.identifier.doi10.1186/s12879-020-05696-y
dc.identifier.issn1471-2334
dc.identifier.urihttps://hdl.handle.net/10037/21805
dc.language.isoengen_US
dc.publisherBMCen_US
dc.relation.journalBMC Infectious Diseases
dc.relation.projectIDNORAD, direktoratet for utviklingssamarbeid: NORHED QZA 0484 RSA 13/0010en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.titleHigh prevalence of multidrug resistant ESBL- and plasmid mediated AmpC-producing clinical isolates of Escherichia coli at Maputo Central Hospital, Mozambiqueen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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