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dc.contributor.authorPrezioso, Carla
dc.contributor.authorCarletti, Raffaella
dc.contributor.authorObregon, Francisco
dc.contributor.authorPiacentini, Francesca
dc.contributor.authorMaricone, Anna Maria
dc.contributor.authorSoda, Giuseppe
dc.contributor.authorMoens, Ugo
dc.contributor.authorDi Gioia, Cira
dc.contributor.authorPietropaolo, Valeria
dc.date.accessioned2021-07-07T10:54:29Z
dc.date.available2021-07-07T10:54:29Z
dc.date.issued2021-01-05
dc.description.abstractBecause the incidence of Merkel cell carcinoma (MCC) has increased significantly during the last 10 years and it is recognized that Merkel cell polyomavirus (MCPyV) and ultraviolet (UV) radiation represent two different etiological inputs sharing clinical, histopathological, and prognostic similar features, although with different prognosis, this study investigated the detection of MCPyV in skin and lymph nodes with histological diagnosis of MCC. Formalin-fixed paraffin-embedded tissue (FFPE) were retrieved from archived specimens and MCPyV non-coding control region (NCCR) and viral capsid protein 1 (VP1) sequences were amplified and sequenced. Results provide an interesting observation concerning the discrepancy between the MCPyV DNA status in primary and metastatic sites: in fact, in all cases in which primary and metastatic lesions were investigated, MCPyV DNA was detected only in the primary lesions. Our data further support the “hit-and-run” theory, also proposed by other authors, and may lead to speculation that in some MCCs the virus is only necessary for the process of tumor initiation and that further mutations may render the tumor independent from the virus. Few point mutations were detected in the NCCR and only silent mutations were observed in the VP1 sequence compared to the MCPyV MCC350 isolate. To unequivocally establish a role of MCPyV in malignancies, additional well-controlled investigations are required, and larger cohorts should be examined.en_US
dc.identifier.citationPrezioso C, Carletti, Obregon, Piacentini, Maricone, Soda, Moens u, Di Gioia, Pietropaolo V. Evaluation of Merkel Cell Polyomavirus DNA in Tissue Samples from italian Patients With Diagnosis of MCC. Viruses. 2021;13en_US
dc.identifier.cristinIDFRIDAID 1865408
dc.identifier.doi10.3390/v13010061
dc.identifier.issn1999-4915
dc.identifier.urihttps://hdl.handle.net/10037/21811
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.relation.journalViruses
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.titleEvaluation of Merkel Cell Polyomavirus DNA in Tissue Samples from italian Patients With Diagnosis of MCCen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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