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dc.contributor.authorHemmingsen, Lisa Myrseth
dc.contributor.authorGiordani, Barbara
dc.contributor.authorPettersen, Ann Kristin
dc.contributor.authorVitali, Beatrice
dc.contributor.authorBasnet, Purusotam
dc.contributor.authorSkalko-Basnet, Natasa
dc.date.accessioned2021-08-13T10:17:33Z
dc.date.available2021-08-13T10:17:33Z
dc.date.issued2021-03-16
dc.description.abstractSuccessful treatment of skin infections requires eradication of biofilms found in up to 90 % of all chronic wounds, causing delayed healing and increased morbidity. We hypothesized that chitosan hydrogel boosts the activity of liposomally-associated membrane active antimicrobials (MAA) and could potentially improve bacterial and biofilm eradication. Therefore, liposomes (∼300 nm) bearing chlorhexidine (CHX; ∼50 μg/mg lipid) as a model MAA were incorporated into chitosan hydrogel. The novel CHX-liposomes-in-hydrogel formulation was optimized for skin therapy. It significantly inhibited the production of nitric oxide (NO) in lipopolysaccharide (LPS)-induced macrophage and almost completely reduced biofilm formation. Moreover, it reduced Staphylococcus aureus and Pseudomonas aeruginosa adherent bacterial cells in biofilm by 64.2–98.1 %. Chitosan hydrogel boosted the anti-inflammatory and antimicrobial properties of CHX.en_US
dc.identifier.citationHemmingsen LM, Giordani B, Pettersen AK, Vitali B, Basnet P, Skalko-Basnet N. Liposomes-in-chitosan hydrogel boosts potential of chlorhexidine in biofilm eradication in vitro. Carbohydrate Polymers. 2021;262:117939en_US
dc.identifier.cristinIDFRIDAID 1899424
dc.identifier.doihttps://doi.org/10.1016/j.carbpol.2021.117939
dc.identifier.issn0144-8617
dc.identifier.issn1879-1344
dc.identifier.urihttps://hdl.handle.net/10037/22034
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.ispartofHemmingsen, L.M. (2022). Advanced topical delivery systems for membrane-active antimicrobials. Exploring nature to improve antimicrobial wound therapy. (Doctoral thesis). <a href=https://hdl.handle.net/10037/27103>https://hdl.handle.net/10037/27103</a>.
dc.relation.journalCarbohydrate Polymers
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Pharmacology: 728en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Farmakologi: 728en_US
dc.titleLiposomes-in-chitosan hydrogel boosts potential of chlorhexidine in biofilm eradication in vitroen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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