Show simple item record

dc.contributor.authorLaidmae, Ivo
dc.contributor.authorMeos, Andres
dc.contributor.authorAlainezhad Kjærvik, Irja
dc.contributor.authorIngebrigtsen, Sveinung Gaarden
dc.contributor.authorSkalko-Basnet, Natasa
dc.contributor.authorKirsimäe, Kalle
dc.contributor.authorRomann, Tavo
dc.contributor.authorJoost, Urmas
dc.contributor.authorKisand, Vambola
dc.contributor.authorKogermann, Karin
dc.date.accessioned2021-11-10T08:40:56Z
dc.date.available2021-11-10T08:40:56Z
dc.date.issued2021-10-20
dc.description.abstractThe hydration of phospholipids, electrospun into polymeric nanofibers and used as templates for liposome formation, offers pharmaceutical advantages as it avoids the storage of liposomes as aqueous dispersions. The objective of the present study was to electrospin and characterize amphiphilic nanofibers as templates for the preparation of antibiotic-loaded liposomes and compare this method with the conventional film-hydration method followed by extrusion. The comparison was based on particle size, encapsulation efficiency and drug-release behavior. Chloramphenicol (CAM) was used at different concentrations as a model antibacterial drug. Phosphatidylcoline (PC) with polyvinylpyrrolidone (PVP), using ethanol as a solvent, was found to be successful in fabricating the amphiphilic composite drug-loaded nanofibers as well as liposomes with both methods. The characterization of the nanofiber templates revealed that fiber diameter did not affect the liposome size. According to the optical microscopy results, the immediate hydration of phospholipids deposited on the amphiphilic nanofibers occurred within a few seconds, resulting in the formation of liposomes in water dispersions. The liposomes appeared to aggregate more readily in the concentrated than in the diluted solutions. The drug encapsulation efficiency for the fiber-hydrated liposomes varied between 14.9 and 28.1% and, for film-hydrated liposomes, between 22.0 and 77.1%, depending on the CAM concentrations and additional extrusion steps. The nanofiber hydration method was faster, as less steps were required for the in-situ liposome preparation than in the film-hydration method. The liposomes obtained using nanofiber hydration were smaller and more homogeneous than the conventional liposomes, but less drug was encapsulated.en_US
dc.identifier.citationLaidmae I, Meos, Alainezhad Kjærvik, Ingebrigtsen SG, Skalko-Basnet N, Kirsimäe K, Romann, Joost U, Kisand, Kogermann K. Electrospun Amphiphilic Nanofibers as Templates for In Situ Preparation of Chloramphenicol-Loaded Liposomes. Pharmaceutics. 2021;13:1742en_US
dc.identifier.cristinIDFRIDAID 1950854
dc.identifier.doihttps://doi.org/10.3390/pharmaceutics13111742
dc.identifier.issn1999-4923
dc.identifier.urihttps://hdl.handle.net/10037/22960
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.relation.journalPharmaceutics
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Pharmacology: 728en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Farmakologi: 728en_US
dc.titleElectrospun Amphiphilic Nanofibers as Templates for In Situ Preparation of Chloramphenicol-Loaded Liposomesen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


File(s) in this item

Thumbnail

This item appears in the following collection(s)

Show simple item record