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dc.contributor.authorSchneider, Yannik Karl-Heinz
dc.contributor.authorAndersen, Jeanette Hammer
dc.contributor.authorHansen, Espen
dc.contributor.authorJørgensen, Solveig M.
dc.date.accessioned2021-11-24T11:38:56Z
dc.date.available2021-11-24T11:38:56Z
dc.date.issued2021-11-01
dc.description.abstractA key step in the process of isolating microbial natural products is the preparation of an extract from a culture. This step determines which molecules will be available for detection in the subsequent chemical and biological analysis of a biodiscovery pipeline. In the present study we wanted to document potential differences in performance between liquid–liquid extraction using ethyl acetate and liquid–solid extraction using a poly‐benzyl‐resin. For the comparison of the two extraction protocols, we spiked a culture of Flavobacterium sp. with a diverse selection of natural products of microbial and plant origin to investigate whether the methods were comparable with respect to selectivity. We also investigated the efficiency of the two extraction methods quantita‐ tively, using water spiked with a selection of natural products, and studied the quantitative effect of different pH levels of the aqueous solutions on the extraction yields of the two methods. The same compounds were extracted by the two methods, but the solid‐phase extract contained more media components compared with the liquid‐phase extract. Quantitatively, the two extraction methods varied in their recovery rates. We conclude that practical aspects could be more important when selecting one of the extraction protocols, as their efficiencies in extracting specific compounds were quite similar.en_US
dc.identifier.citationSchneider, Jørgensen S, Andersen, Hansen. Qualitative and Quantitative Comparison of Liquid–Liquid Phase Extraction Using Ethyl Acetate and Liquid–Solid Phase Extraction Using Poly-Benzyl-Resin for Natural Products. Applied Sciences. 2021;11en_US
dc.identifier.cristinIDFRIDAID 1950370
dc.identifier.doi10.3390/app112110241
dc.identifier.issn2076-3417
dc.identifier.urihttps://hdl.handle.net/10037/23151
dc.language.isoengen_US
dc.relation.journalApplied Sciences
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.subjectVDP::Agriculture and fishery disciplines: 900::Fisheries science: 920en_US
dc.subjectVDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920en_US
dc.titleQualitative and Quantitative Comparison of Liquid–Liquid Phase Extraction Using Ethyl Acetate and Liquid–Solid Phase Extraction Using Poly-Benzyl-Resin for Natural Productsen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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