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dc.contributor.authorKang, Wenjing
dc.contributor.authorFromm, Bastian
dc.contributor.authorHouben, Anna J.
dc.contributor.authorHøye, Eirik
dc.contributor.authorBezdan, Daniela
dc.contributor.authorArnan, Carme
dc.contributor.authorThrane, Kim
dc.contributor.authorAsp, Michaela
dc.contributor.authorJohnson, Rory
dc.contributor.authorBiryukova, Inna
dc.contributor.authorFriedländer, Marc R.
dc.date.accessioned2021-12-28T11:13:46Z
dc.date.available2021-12-28T11:13:46Z
dc.date.issued2021-11-16
dc.description.abstractPrevious large-scale studies have uncovered many features that determine the processing of microRNA (miRNA) precursors; however, they have been conducted in vitro. Here, we introduce MapToCleave, a method to simultaneously profile processing of thousands of distinct RNA structures in living cells. We find that miRNA precursors with a stable lower basal stem are more efficiently processed and also have higher expression in vivo in tissues from 20 animal species. We systematically compare the importance of known and novel sequence and structural features and test biogenesis of miRNA precursors from 10 animal and plant species in human cells. Lastly, we provide evidence that the GHG motif better predicts processing when defined as a structure rather than sequence motif, consistent with recent cryogenic electron microscopy (cryo-EM) studies. In summary, we apply a screening assay in living cells to reveal the importance of lower basal stem stability for miRNA processing and in vivo expression.en_US
dc.identifier.citationKang, Fromm, Houben, Høye, Bezdan, Arnan, Thrane, Asp, Johnson, Biryukova, Friedländer. MapToCleave: High-throughput profiling of microRNA biogenesis in living cells. Cell reports. 2021;37(7)en_US
dc.identifier.cristinIDFRIDAID 1963592
dc.identifier.doi10.1016/j.celrep.2021.110015
dc.identifier.issn2211-1247
dc.identifier.urihttps://hdl.handle.net/10037/23520
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalCell reports
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/758397/Norway/MicroRNA functions in single cells/miRCell/en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.titleMapToCleave: High-throughput profiling of microRNA biogenesis in living cellsen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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