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dc.contributor.authorAcuna Maldonado, Sebastian Andres
dc.contributor.authorROY, MAYANK
dc.contributor.authorVillegas, Luis
dc.contributor.authorDubey, Vishesh Kumar
dc.contributor.authorAhluwalia, Balpreet Singh
dc.contributor.authorAgarwal, Krishna
dc.date.accessioned2022-01-31T12:06:43Z
dc.date.available2022-01-31T12:06:43Z
dc.date.issued2021-08-11
dc.description.abstractContrast in fluorescence microscopy images allows for the differentiation between different structures by their difference in intensities. However, factors such as point-spread function and noise may reduce it, affecting its interpretability. We identified that fluctuation of emitters in a stack of images can be exploited to achieve increased contrast when compared to the average and Richardson-Lucy deconvolution. We tested our methods on four increasingly challenging samples including tissue, in which case results were comparable to the ones obtained by structured illumination microscopy in terms of contrast.en_US
dc.identifier.citationAcuna Maldonado, ROY, Villegas, Dubey, Ahluwalia, Agarwal. Deriving high contrast fluorescence microscopy images through low contrast noisy image stacks. Biomedical Optics Express. 2021en_US
dc.identifier.cristinIDFRIDAID 1985211
dc.identifier.doi10.1364/BOE.422747
dc.identifier.issn2156-7085
dc.identifier.urihttps://hdl.handle.net/10037/23845
dc.language.isoengen_US
dc.publisherOptical Society of Americaen_US
dc.relation.ispartofAcuna Maldonado, S.A. (2023). Multiple Signal Classification Algorithm: A computational microscopy tool for fluorescence microscopy. (Doctoral thesis). <a href=https://hdl.handle.net/10037/31879>https://hdl.handle.net/10037/31879</a>.
dc.relation.journalBiomedical Optics Express
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/FRINATEK/288082/Norway/Label-free nanoscopy of living cells through nanoscale refractive index profiler//en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/?/804233?/Norway/?/?/en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/749666/Norway/Chip-based MUSICAL nanoscopy for imaging endocytosis pathways of phage viruses in liver sinusoidal endothelial cells/MUSICAL/en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.titleDeriving high contrast fluorescence microscopy images through low contrast noisy image stacksen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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