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dc.contributor.authorSzafranska, Karolina
dc.contributor.authorHolte, Christopher Florian
dc.contributor.authorKruse, Larissa Dorothea
dc.contributor.authorMao, Hong
dc.contributor.authorØie, Cristina Ionica
dc.contributor.authorSymonski, Marek
dc.contributor.authorZapotoczny, Bartlomiej
dc.contributor.authorMcCourt, Peter Anthony
dc.date.accessioned2022-02-24T13:31:12Z
dc.date.available2022-02-24T13:31:12Z
dc.date.issued2021-07-28
dc.description.abstractLiver Sinusoidal Endothelial Cells (LSEC) line the hepatic vasculature providing blood filtration via transmembrane nanopores called fenestrations. These structures are 50−300 nm in diameter, which is below the resolution limit of a conventional light microscopy. To date, there is no standardized method of fenestration image analysis. With this study, we provide and compare three different approaches: manual measurements, a semi-automatic (threshold-based) method, and an automatic method based on user-friendly open source machine learning software. Images were obtained using three super resolution techniques – atomic force microscopy (AFM), scanning electron microscopy (SEM), and structured illumination microscopy (SIM). Parameters describing fenestrations such as diameter, area, roundness, frequency, and porosity were measured. Finally, we studied the user bias by comparison of the data obtained by five different users applying provided analysis methods.en_US
dc.identifier.citationSzafranska K, Holte C, Kruse LD, Mao H, Øie CI, Symonski, Zapotoczny B, McCourt PAG. Quantitative analysis methods for studying fenestrations in liver sinusoidal endothelial cells. A comparative study. Micron. 2021;150:103121en_US
dc.identifier.cristinIDFRIDAID 1982337
dc.identifier.doi10.1016/j.micron.2021.103121
dc.identifier.issn0968-4328
dc.identifier.issn1878-4291
dc.identifier.urihttps://hdl.handle.net/10037/24137
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.ispartofSzafranska, K. (2022). Novel screening methods for nanoscale changes in liver cell fenestrations elicited by pharmaceuticals. (Doctoral thesis). <a href=https://hdl.handle.net/10037/25981>https://hdl.handle.net/10037/25981</a>.
dc.relation.ispartofHolte, C.F. (2024). Novel insights into the fenestrated scavenger endothelium of the liver sinusoid. (Doctoral thesis). <a href=https://hdl.handle.net/10037/33068>https://hdl.handle.net/10037/33068</a>
dc.relation.journalMicron
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/766181/Norway/Super-resolution optical microscopy of nanosized pore dynamics in endothelial cells/DeLIVER/en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.titleQuantitative analysis methods for studying fenestrations in liver sinusoidal endothelial cells. A comparative studyen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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