dc.contributor.author | Nilsson, Per | |
dc.contributor.author | Pettersen, Kristin | |
dc.contributor.author | Oppermann, Martin | |
dc.contributor.author | Skjeflo, Espen Waage | |
dc.contributor.author | Fure, Hilde | |
dc.contributor.author | Christiansen, Dorte | |
dc.contributor.author | Mollnes, Tom Eirik | |
dc.date.accessioned | 2022-03-01T12:44:14Z | |
dc.date.available | 2022-03-01T12:44:14Z | |
dc.date.issued | 2021-04-14 | |
dc.description.abstract | Enzyme-linked immunosorbent assay (ELISA) enables fast and simple quantification of analytes in the pico- to nanogram range in complex samples. Here, we describe an ELISA for the detection of porcine C3a as a marker for complement activation. Antibody specificity is critical for a robust assay. This assay is based on a pair of antibodies specific for the porcine C3a molecule and thus does not react with native C3. | en_US |
dc.description | This version of the article has been accepted for publication, after peer review and is subject to Springer Nature’s <a href=https://www.springernature.com/gp/open-research/policies/accepted-manuscript-terms>AM terms of use</a>, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at <a href=http://dx.doi.org/10.1007/978-1-0716-1016-9_5>http://dx.doi.org/10.1007/978-1-0716-1016-9_5</a>. | en_US |
dc.identifier.citation | Nilsson P, Pettersen K, Oppermann, Skjeflo EW, Fure H, Christiansen D, Mollnes TE. Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope-Based Enzyme-Linked Immunosorbent Assay. Methods in molecular biology. 2021;2227:51-59 | en_US |
dc.identifier.cristinID | FRIDAID 1994727 | |
dc.identifier.doi | 10.1007/978-1-0716-1016-9_5 | |
dc.identifier.issn | 1064-3745 | |
dc.identifier.issn | 1940-6029 | |
dc.identifier.uri | https://hdl.handle.net/10037/24203 | |
dc.language.iso | eng | en_US |
dc.publisher | Springer Nature | en_US |
dc.relation.journal | Methods in molecular biology | |
dc.relation.projectID | Norges forskningsråd: 223255 | en_US |
dc.rights.accessRights | openAccess | en_US |
dc.rights.holder | Copyright 2021 Springer Nature | en_US |
dc.subject | VDP::Mathematics and natural science: 400::Basic biosciences: 470::Biochemistry: 476 | en_US |
dc.subject | VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Biokjemi: 476 | en_US |
dc.title | Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope-Based Enzyme-Linked Immunosorbent Assay | en_US |
dc.type.version | acceptedVersion | en_US |
dc.type | Journal article | en_US |
dc.type | Tidsskriftartikkel | en_US |
dc.type | Peer reviewed | en_US |