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dc.contributor.authorFostervold, Aasmund
dc.contributor.authorHetland, Marit Andrea Klokkhammer
dc.contributor.authorBakksjø, Ragna-Johanne
dc.contributor.authorBernhoff, Eva
dc.contributor.authorHolt, Kathryn E.
dc.contributor.authorSamuelsen, Ørjan
dc.contributor.authorSimonsen, Gunnar Skov
dc.contributor.authorSundsfjord, Arnfinn
dc.contributor.authorWyres, Kelly L.
dc.contributor.authorLöhr, Iren Høyland
dc.date.accessioned2022-03-07T10:41:30Z
dc.date.available2022-03-07T10:41:30Z
dc.date.issued2021-12-22
dc.description.abstractObjectives: To use the nationwide Norwegian surveillance programme on resistant microbes in humans (NORM) to address longitudinal changes in the population structure of Klebsiella pneumoniae isolates from 2001–15, focusing on the emergence and dissemination of ESBL-producing K. pneumoniae in Norway.<p> <p>Methods: Among blood (n = 6124) and urinary tract (n = 5496) surveillance isolates from 2001–15, we used Illumina technology to whole genome sequence 201 ESBL-producing isolates from blood (n = 130) and urine (n = 71), and 667 non-ESBL isolates from blood. Complete genomes for four isolates were resolved with Oxford Nanopore sequencing.<p> <p>Results: In a highly diverse collection, Klebsiella variicola ssp. variicola caused 24.5% of Klebsiella pneumoniae species complex (KpSC) bacteraemias. ESBL production was limited to K. pneumoniae sensu stricto (98.5%). A diverse ESBL population of 57 clonal groups (CGs) were dominated by MDR CG307 (17%), CG15 (12%), CG70 (6%), CG258 (5%) and CG45 (5%) carrying bla<sub>CTX-M-15</sub>. Yersiniabactin was significantly more common in ESBL-positive (37.8%) compared with non-ESBL K. pneumoniae sensu stricto isolates (12.7%), indicating convergence of virulence and resistance determinants. Moreover, we found a significantly lower prevalence of yersiniabactin (3.0%, 37.8% and 17.3%), IncFIB (58.7%, 87.9% and 79.4%) and IncFII plasmid replicons (40.5%, 82.8% and 54.2%) in K. variicola ssp. variicola compared with ESBL- and non-ESBL K. pneumoniae sensu stricto isolates, respectively.<p> <p>Conclusions: The increase in Norwegian ESBL-producing KpSC during 2010–15 was driven by CG307 and CG15 carrying bla<sub>CTX-M-15</sub>. K. variicola ssp. variicola was a frequent cause of invasive KpSC infection, but rarely carried ESBLsen_US
dc.identifier.citationFostervold A, Hetland MAK, Bakksjø R, Bernhoff E, Holt KE, Samuelsen Ø, Simonsen GS, Sundsfjord A, Wyres KL, Löhr IH. A nationwide genomic study of clinical Klebsiella pneumoniae in Norway 2001–15: introduction and spread of ESBLs facilitated by clonal groups CG15 and CG307. Journal of Antimicrobial Chemotherapy. 2021en_US
dc.identifier.cristinIDFRIDAID 2006164
dc.identifier.doi10.1093/jac/dkab463
dc.identifier.issn0305-7453
dc.identifier.issn1460-2091
dc.identifier.urihttps://hdl.handle.net/10037/24296
dc.language.isoengen_US
dc.publisherOxford University Pressen_US
dc.relation.journalJournal of Antimicrobial Chemotherapy
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.titleA nationwide genomic study of clinical Klebsiella pneumoniae in Norway 2001–15: introduction and spread of ESBLs facilitated by clonal groups CG15 and CG307en_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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