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dc.contributor.authorKnudsen, Steen Wilhelm
dc.contributor.authorHesselsøe, Martin
dc.contributor.authorThaulow, Jens
dc.contributor.authorAgersnap, Sune
dc.contributor.authorHansen, Brian Klitgaard
dc.contributor.authorJacobsen, Magnus Wulff
dc.contributor.authorBekkevold, Dorte
dc.contributor.authorJensen, Søren K.S.
dc.contributor.authorMøller, Peter Daniel Rask
dc.contributor.authorAndersen, Jesper Harbo
dc.date.accessioned2022-03-22T09:57:51Z
dc.date.available2022-03-22T09:57:51Z
dc.date.issued2022-01-14
dc.description.abstractMonitoring the distribution of marine non-indigenous species is a challenging task. To support this monitoring, we developed and validated the specificity of 12 primer-probe assays for detection of environmental DNA (eDNA) from marine species all non-indigenous to Europe. The species include sturgeons, a Pacific red algae, oyster thief, a freshwater hydroid from the Black Sea, Chinese mitten crab, Pacific oyster, warty comb jelly, sand gaper, round goby, pink salmon, rainbow trout and North American mud crab. We tested all assays in the laboratory, on DNA extracted from both the target and non-target species to ensure that they only amplified DNA from the intended species. Subsequently, all assays were used to analyse water samples collected at 16 different harbours across two different seasons during 2017. We also included six previously published assays targeting eDNA from goldfish, European carp, two species of dinoflagellates of the genera Karenia and Prorocentrum, two species of the heterokont flagellate genus Pseudochattonella. Conventional monitoring was carried out alongside eDNA sampling but with only one sampling event over the one year. Because eDNA was relatively fast and easy to collect compared to conventional sampling, we sampled eDNA twice during 2017, which showed seasonal changes in the distribution of non-indigenous species. Comparing eDNA levels with salinity gradients did not show any correlation. A significant correlation was observed between number of species detected with conventional monitoring methods and number of species found using eDNA at each location. This supports the use of eDNA for surveillance of the distribution of marine non-indigenous species, where the speed and relative easy sampling in the field combined with fast molecular analysis may provide advantages compared to conventional monitoring methods. Prior validation of assays increases taxonomic precision, and laboratorial setup facilitates analysis of multiple samples simultaneously. The specific eDNA assays presented here can be implemented directly in monitoring programmes across Europe and potentially worldwide to infer a more precise picture of the dynamics in the distribution of marine non-indigenous species.en_US
dc.identifier.citationKnudsen SW, Hesselsøe M, Thaulow J, Agersnap S, Hansen BK, Jacobsen, Bekkevold D, Jensen, Møller PDR, Andersen JH. Monitoring of environmental DNA from nonindigenous species of algae, dinoflagellates and animals in the North East Atlantic. Science of the Total Environment. 2022;821en_US
dc.identifier.cristinIDFRIDAID 1990189
dc.identifier.doi10.1016/j.scitotenv.2022.153093
dc.identifier.issn0048-9697
dc.identifier.issn1879-1026
dc.identifier.urihttps://hdl.handle.net/10037/24479
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalScience of the Total Environment
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2022 The Author(s)en_US
dc.titleMonitoring of environmental DNA from nonindigenous species of algae, dinoflagellates and animals in the North East Atlanticen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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