Show simple item record

dc.contributor.authorHaldorsen, Bjørg
dc.contributor.authorGiske, Christian G
dc.contributor.authorHansen, Dennis S
dc.contributor.authorHelgason, Kristjan Orri
dc.contributor.authorKahlmeter, Gunnar
dc.contributor.authorLöhr, Iren Høyland
dc.contributor.authorMatuschek, Erika
dc.contributor.authorÖsterblad, Monica
dc.contributor.authorRantakokko-Jalava, Kaisu
dc.contributor.authorWang, Mikala
dc.contributor.authorSmåbrekke, Lars
dc.contributor.authorSamuelsen, Ørjan
dc.contributor.authorSundsfjord, Arnfinn
dc.date.accessioned2022-04-27T10:43:10Z
dc.date.available2022-04-27T10:43:10Z
dc.date.issued2018-07-24
dc.description.abstractObjectives: To examine performance of EUCAST disc diffusion and supplementary MIC methods for detection of Enterobacteriaceae with reduced susceptibility to meropenem using EUCAST screening recommendations.<p> <p>Methods: Sixty-one Nordic laboratories delivered data on EUCAST disc diffusion (n " 61), semi-automated meropenem MIC (n " 23; VITEK2, n " 20 and Phoenix, n " 3) and gradient meropenem MIC (n " 58) methods. The strains (n " 27) included the major carbapenemase classes (A, n " 4; B, n " 9; D, n " 6) involved in the global spread of carbapenemase-producing Enterobacteriaceae (CPE) and non-CPE strains (n " 8) covering a range of broth microdilution (BMD) meropenem MICs.<p> <p>Results: A triplicate Klebsiella variicola (meropenem MIC 0.5 mg/L) harbouring OXA-48 and Escherichia coli ATCC 25922 showed an overall good precision. Meropenem zone diameters below the EUCAST screening cut-off (,27 mm) were reported for strains with MIC 1 mg/L (n " 21), irrespective of resistance mechanism. For three strains (MIC " 0.5 mg/L) with OXA-48/-181, eight laboratories provided meropenem zone diameters above the screening cut-off. Very major errors (VMEs) were not observed. The overall distributions of major errors (MEs) and minor errors (mEs) were 9% and 36% (disc diffusion), 26% and 18% (VITEK2) and 7% and 20% (gradient MIC), respectively. Differences in ME and mE distributions between disc diffusion and MIC gradient tests compared with semi-automated methods were significant (P , 0.0001), using BMD MICs as a reference for categorization.<p> <p>Conclusions: The EUCAST disc diffusion method is a robust method to screen for CPE but isolates with meropenem MICs ,1 mg/L pose challenges. The high ME rate in semi-automated methods might deter appropriate use of carbapenems in CPE infections with limited therapeutic options.en_US
dc.identifier.citationHaldorsen BC, Giske C, Hansen, Helgason, Kahlmeter G, Löhr IH, Matuschek E, Österblad, Rantakokko-Jalava K, Wang M, Småbrekke L, Samuelsen Ø, Sundsfjord A. Performance of the EUCAST disc diffusion method and two MIC methods in detection of Enterobacteriaceae with reduced susceptibility to meropenem: the NordicAST CPE study.. Journal of Antimicrobial Chemotherapy. 2018;73(10):2738-2747
dc.identifier.cristinIDFRIDAID 1608771
dc.identifier.doi10.1093/jac/dky276
dc.identifier.issn0305-7453
dc.identifier.issn1460-2091
dc.identifier.urihttps://hdl.handle.net/10037/24908
dc.language.isoengen_US
dc.publisherOxford University Pressen_US
dc.relation.journalJournal of Antimicrobial Chemotherapy
dc.rights.holderCopyright 2018 The Author(s)en_US
dc.titlePerformance of the EUCAST disc diffusion method and two MIC methods in detection of Enterobacteriaceae with reduced susceptibility to meropenem: the NordicAST CPE study.en_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


File(s) in this item

Thumbnail

This item appears in the following collection(s)

Show simple item record