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dc.contributor.authorPurohit, Amit Anand
dc.contributor.authorJohansen, Jostein a
dc.contributor.authorHansen, Hilde
dc.contributor.authorLeiros, Hanna-Kirsti S.
dc.contributor.authorKashulin, Alexander
dc.contributor.authorKarlsen, Christian
dc.contributor.authorSmalås, Arne O.
dc.contributor.authorHaugen, Peik
dc.contributor.authorWillassen, Nils Peder
dc.date.accessioned2022-06-27T06:41:13Z
dc.date.available2022-06-27T06:41:13Z
dc.date.issued2013-05-31
dc.description.abstractAims: The aim of this study was to use a sensitive method to screen and quantify 57 Vibrionaceae strains for the production of acyl-homoserine lactones (AHLs) and map the resulting AHL profiles onto a host phylogeny.<p> <p>Methods and Results: We used a high-performance liquid chromatography– tandem mass spectrometry (HPLC-MS/MS) protocol to measure AHLs in spent media after bacterial growth. First, the presence/absence of AHLs (qualitative analysis) was measured to choose internal standard for subsequent quantitative AHL measurements. We screened 57 strains from three genera (Aliivibrio, Photobacterium and Vibrio) of the same family (i.e. Vibrionaceae). Our results show that about half of the isolates produced multiple AHLs, typically at 25–5000 nmol l<sup>-1</sup> . <p>Conclusions: This work shows that production of AHL quorum sensing signals is found widespread among Vibrionaceae bacteria and that closely related strains typically produce similar AHL profiles. <p>Significance and Impact of the Study: The AHL detection protocol presented in this study can be applied to a broad range of bacterial samples and may contribute to a wider mapping of AHL production in bacteria, for example, in clinically relevant strains.en_US
dc.identifier.citationPurohit A, Johansen Ja, Hansen H, Leiros H, Kashulin A, Karlsen C, Smalås A, Haugen P, Willassen NP. Presence of acyl-homoserine lactones in 57 members of the Vibrionaceae family. Journal of Applied Microbiology. 2013;115(3):835-847en_US
dc.identifier.cristinIDFRIDAID 1057779
dc.identifier.doi10.1111/jam.12264
dc.identifier.issn1364-5072
dc.identifier.issn1365-2672
dc.identifier.urihttps://hdl.handle.net/10037/25570
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.journalJournal of Applied Microbiology
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2013 The Author(s)en_US
dc.titlePresence of acyl-homoserine lactones in 57 members of the Vibrionaceae familyen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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