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dc.contributor.authorDiaz Cánova, Diana Karina
dc.contributor.authorMoens, Ugo
dc.contributor.authorBrinkmann, Annika
dc.contributor.authorNitsche, Andreas
dc.contributor.authorOkeke, malachy ifeanyi
dc.date.accessioned2022-11-14T12:33:48Z
dc.date.available2022-11-14T12:33:48Z
dc.date.issued2022-05-03
dc.description.abstractOrthopoxviruses (OPXVs) not only infect their natural hosts, but some OPXVs can also cause disease in humans. Previously, we partially characterized an OPXV isolated from an 18-year-old male living in Northern Norway. Restriction enzyme analysis and partial genome sequencing characterized this virus as an atypical cowpox virus (CPXV), which we named CPXV-No-H2. In this study, we determined the complete genome sequence of CPXV-No-H2 using Illumina and Nanopore sequencing. Our results showed that the whole CPXV-No-H2 genome is 220,276 base pairs (bp) in length, with inverted terminal repeat regions of approximately 7 kbp, containing 217 predicted genes. Seventeen predicted CPXV-No-H2 proteins were most similar to OPXV proteins from the Old World, including Ectromelia virus (ECTV) and Vaccinia virus, and North America, Alaskapox virus (AKPV). CPXV-No-H2 has a mosaic genome with genes most similar to other OPXV genes, and seven potential recombination events were identified. The phylogenetic analysis showed that CPXV-No-H2 formed a separate clade with the German CPXV isolates CPXV_GerMygEK938_17 and CPXV_Ger2010_MKY, sharing 96.4 and 96.3% nucleotide identity, respectively, and this clade clustered closely with the ECTV-OPXV Abatino clade. CPXV-No-H2 is a mosaic virus that may have arisen out of several recombination events between OPXVs, and its phylogenetic clustering suggests that ECTV-Abatino-like cowpox viruses form a distinct, new clade of cowpox viruses.en_US
dc.identifier.citationDiaz Cánova, Moens, Brinkmann, Nitsche, Okeke. Genomic Sequencing and Analysis of a Novel Human Cowpox Virus With Mosaic Sequences From North America and Old World Orthopoxvirus. Frontiers in Microbiology. 2022;13en_US
dc.identifier.cristinIDFRIDAID 2045889
dc.identifier.doi10.3389/fmicb.2022.868887
dc.identifier.issn1664-302X
dc.identifier.urihttps://hdl.handle.net/10037/27360
dc.language.isoengen_US
dc.publisherFrontiers Mediaen_US
dc.relation.ispartofDiaz-Cánova, D. (2023). Evolutionary genomics of cowpox virus and recombination in vitro between a naturally occurring cowpox virus and a vaccinia virus vectored influenza vaccine. (Doctoral thesis). <a href=https://hdl.handle.net/10037/29180>https://hdl.handle.net/10037/29180</a>.
dc.relation.journalFrontiers in Microbiology
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2022 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleGenomic Sequencing and Analysis of a Novel Human Cowpox Virus With Mosaic Sequences From North America and Old World Orthopoxvirusen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)