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dc.contributor.authorNorreen-Thorsen, Marthe
dc.contributor.authorStruck, Eike Christopher
dc.contributor.authorÖling, Sofia Maria
dc.contributor.authorZwahlen, Martin
dc.contributor.authorVon Feilitzen, Kalle
dc.contributor.authorOdeberg, Jacob
dc.contributor.authorLindskog, Cecilia
dc.contributor.authorPontén, Fredrik
dc.contributor.authorUhlén, Mathias
dc.contributor.authorDusart, Philip James
dc.contributor.authorButler, Lynn
dc.date.accessioned2022-11-15T07:51:22Z
dc.date.available2022-11-15T07:51:22Z
dc.date.issued2022-07-12
dc.description.abstractThe importance of defining cell-type-specific genes is well acknowledged. Technological advances facilitate high-resolution sequencing of single cells, but practical challenges remain. Adipose tissue is composed primarily of adipocytes, large buoyant cells requiring extensive, artefact-generating processing for separation and analysis. Thus, adipocyte data are frequently absent from single-cell RNA sequencing (scRNA-seq) datasets, despite being the primary functional cell type. Here, we decipher cell-type-enriched transcriptomes from unfractionated human adipose tissue RNA-seq data. We profile all major constituent cell types, using 527 visceral adipose tissue (VAT) or 646 subcutaneous adipose tissue (SAT) samples, identifying over 2,300 cell-type-enriched transcripts. Sex-subset analysis uncovers a panel of male-only cell-type-enriched genes. By resolving expression profiles of genes differentially expressed between SAT and VAT, we identify mesothelial cells as the primary driver of this variation. This study provides an accessible method to profile cell-type-enriched transcriptomes using bulk RNA-seq, generating a roadmap for adipose tissue biology.en_US
dc.identifier.citationNorreen-Thorsen, Struck, Öling, Zwahlen, Von Feilitzen, Odeberg, Lindskog, Pontén, Uhlén, Dusart, Butler. A human adipose tissue cell-type transcriptome atlas. Cell reports. 2022;40(2)en_US
dc.identifier.cristinIDFRIDAID 2058418
dc.identifier.doi10.1016/j.celrep.2022.111046
dc.identifier.issn2211-1247
dc.identifier.urihttps://hdl.handle.net/10037/27364
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.ispartofNorreen-Thorsen, M. (2023). The identification of cell type defining genes across human tissues and the functional study of the endothelial adhesion G protein-coupled receptor L4. (Doctoral thesis). <a href=https://hdl.handle.net/10037/29399>https://hdl.handle.net/10037/29399</a>
dc.relation.ispartofStruck, E.C. (2023). The Endothelial Cell Response to Inflammation, the Functional Role of the Endothelial-enriched Protein KANK3 and the Adipose Tissue Transcriptome. (Doctoral thesis). <a href=https://hdl.handle.net/10037/31501>https://hdl.handle.net/10037/31501</a>.
dc.relation.journalCell reports
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2022 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleA human adipose tissue cell-type transcriptome atlasen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)