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dc.contributor.authorHøye, Eirik
dc.contributor.authorFromm, Bastian
dc.contributor.authorBöttger, Paul H.M.
dc.contributor.authorDomanska, Diana
dc.contributor.authorKristensen, Annette Torgunrud
dc.contributor.authorLund-Andersen, Christin
dc.contributor.authorAbrahamsen, Torveig Weum
dc.contributor.authorFretland, Åsmund Avdem
dc.contributor.authorDagenborg, Vegar Johansen
dc.contributor.authorLorenz, Susanne
dc.contributor.authorEdwin, Bjørn von Gohren
dc.contributor.authorHovig, Eivind
dc.contributor.authorFlatmark, Kjersti
dc.date.accessioned2022-11-24T09:07:39Z
dc.date.available2022-11-24T09:07:39Z
dc.date.issued2022-01-14
dc.description.abstractAlthough microRNAs (miRNAs) contribute to all hallmarks of cancer, miRNA dysregulation in metastasis remains poorly understood. The aim of this work was to reliably identify miRNAs associated with metastatic progression of colorectal cancer (CRC) using novel and previously published nextgeneration sequencing (NGS) datasets generated from 268 samples of primary (pCRC) and metastatic CRC (mCRC; liver, lung and peritoneal metastases) and tumor adjacent tissues. Differential expression analysis was performed using a meticulous bioinformatics pipeline, including only bona fide miRNAs, and utilizing miRNA-tailored quality control and processing. Five miRNAs were identified as upregulated at multiple metastatic sites Mir-210 3p, Mir191 5p, Mir-8-P1b 3p [mir-141–3p], Mir-1307 5p and Mir-155 5p. Several have previously been implicated in metastasis through involvement in epithelial-tomesenchymal transition and hypoxia, while other identified miRNAs represent novel findings. The use of a publicly available pipeline facilitates reproducibility and allows new datasets to be added as they become available. The set of miRNAs identified here provides a reliable starting-point for further research into the role of miRNAs in metastatic progression.en_US
dc.identifier.citationHøye, Fromm, Böttger, Domanska, Kristensen, Lund-Andersen, Abrahamsen, Fretland, Dagenborg, Lorenz, Edwin, Hovig, Flatmark. A comprehensive framework for analysis of microRNA sequencing data in metastatic colorectal cancer. NAR Cancer. 2022;4(1):1-13en_US
dc.identifier.cristinIDFRIDAID 2077209
dc.identifier.doi10.1093/narcan/zcab051
dc.identifier.issn2632-8674
dc.identifier.urihttps://hdl.handle.net/10037/27510
dc.language.isoengen_US
dc.publisherOxford University Pressen_US
dc.relation.journalNAR Cancer
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2022 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleA comprehensive framework for analysis of microRNA sequencing data in metastatic colorectal canceren_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)