Show simple item record

dc.contributor.authorØstnes Hansen, Kine
dc.contributor.authorHansen, Ida Kristine Østnes
dc.contributor.authorUllsten-Wahlund, Sara
dc.contributor.authorJenssen, Marte
dc.contributor.authorIsaksson, Johan Mattias
dc.contributor.authorLi, Chun
dc.contributor.authorSchneider, Yannik K.-H.
dc.date.accessioned2023-01-11T09:44:42Z
dc.date.available2023-01-11T09:44:42Z
dc.date.issued2022-12-13
dc.description.abstractIsolation of bioactive products from the marine environment is considered a very promising approach to identify new compounds that can be used for further drug development. In this work we have isolated three new compounds from the purpuroine family by mass-guided preparative HPLC; purpuroine K-M. These compounds where screened for antibacterial- and antifungal activity, antibiofilm formation and anti-cell proliferation activity. Additionally, apoptosis-, cell cycle-, kinase binding- and docking studies were performed to evaluate the mechanism-of-action. None of the compounds showed activity in antibacterial-, antibiofilm- or antifungal assays. However, one of the isolated compounds, purpuroine K, showed activity against two cell lines, MV-4-11 and MOLM-13, two AML cell lines both carrying the FTL3-ITD mutation. In MV-4-11 cells, purpuroine K was found to increase apoptosis and arrest cells cycle in G1/G0, which is a common feature of FLT3 inhibitors. Interactions between purpuroine K and the FLT3 wild type or FLT3 ITD mutant proteins could however not be elucidated in our kinase binding and docking studies. In conclusion, we have isolated three novel molecules, purpuroine K-M, one of which (purpuroine K) shows a potent activity against FLT3-ITD mutated AML cell lines, however, the molecular target(s) of purpuroine K still need to be further investigated.en_US
dc.identifier.citationØstnes Hansen, Hansen, Ullsten-Wahlund, Jenssen, Petit, Isaksson. Identification of New Purpuroine Analogues from the Arctic Echinodermata Pteraster militaris That Inhibit FLT3-ITD+ AML Cell Lines. International Journal of Molecular Sciences. 2022en_US
dc.identifier.cristinIDFRIDAID 2103455
dc.identifier.doi10.3390/ijms232415852
dc.identifier.issn1661-6596
dc.identifier.issn1422-0067
dc.identifier.urihttps://hdl.handle.net/10037/28137
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.relation.journalInternational Journal of Molecular Sciences
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2022 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleIdentification of New Purpuroine Analogues from the Arctic Echinodermata Pteraster militaris That Inhibit FLT3-ITD+ AML Cell Linesen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


File(s) in this item

Thumbnail

This item appears in the following collection(s)

Show simple item record

Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)