dc.contributor.author | Kasheverov, Igor E | |
dc.contributor.author | Logashina, Yulia A. | |
dc.contributor.author | Kornilov, Fedor D | |
dc.contributor.author | Lushpa, Vladislav A | |
dc.contributor.author | Maleeva, Ekaterina E | |
dc.contributor.author | Korolkova, Yuliya V. | |
dc.contributor.author | Yu, Jinpeng | |
dc.contributor.author | Zhu, Xiaopeng | |
dc.contributor.author | Zhangsun, Dongting | |
dc.contributor.author | Luo, Sulan | |
dc.contributor.author | Stensvåg, Klara | |
dc.contributor.author | Kudryavtsev, Denis S | |
dc.contributor.author | Mineev, Konstantin S. | |
dc.contributor.author | Andreev, Yaroslav A. | |
dc.date.accessioned | 2023-03-21T06:21:55Z | |
dc.date.available | 2023-03-21T06:21:55Z | |
dc.date.issued | 2022-12-30 | |
dc.description.abstract | Nicotinic acetylcholine receptors (nAChRs) play an important role in the functioning of
the central and peripheral nervous systems, and other organs of living creatures. There are several
subtypes of nAChRs, and almost all of them are considered as pharmacological targets in different
pathological states. The crude venom of the sea anemone Metridium senile showed the ability to
interact with nAChRs. Four novel peptides (Ms11a-1–Ms11a-4) with nAChR binding activity were
isolated. These peptides stabilized by three disulfide bridges have no noticeable homology with
any known peptides. Ms11a-1–Ms11a-4 showed different binding activity towards the muscle-type
nAChR from the Torpedo californica ray. The study of functional activity and selectivity for the most
potent peptide (Ms11a-3) revealed the highest antagonism towards the heterologous rat α9α10
nAChR compared to the muscle and α7 receptors. Structural NMR analysis of two toxins (Ms11a-2
and Ms11a-3) showed that they belong to a new variant of the inhibitor cystine knot (ICK) fold but
have a prolonged loop between the fifth and sixth cysteine residues. Peptides Ms11a-1–Ms11a-4
could represent new pharmacological tools since they have structures different from other known
nAChRs inhibitors. | en_US |
dc.identifier.citation | Kasheverov, Logashina YA, Kornilov, Lushpa, Maleeva, Korolkova YV, Yu, Zhu X, Zhangsun, Luo, Stensvåg K, Kudryavtsev, Mineev KS, Andreev YA. Peptides from the Sea Anemone Metridium senile with Modified Inhibitor Cystine Knot (ICK) Fold Inhibit Nicotinic Acetylcholine Receptors. Toxins. 2022;15(1) | en_US |
dc.identifier.cristinID | FRIDAID 2134973 | |
dc.identifier.doi | 10.3390/toxins15010028 | |
dc.identifier.issn | 2072-6651 | |
dc.identifier.uri | https://hdl.handle.net/10037/28800 | |
dc.language.iso | eng | en_US |
dc.publisher | MDPI | en_US |
dc.relation.journal | Toxins | |
dc.rights.accessRights | openAccess | en_US |
dc.rights.holder | Copyright 2022 The Author(s) | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | en_US |
dc.rights | Attribution 4.0 International (CC BY 4.0) | en_US |
dc.title | Peptides from the Sea Anemone Metridium senile with Modified Inhibitor Cystine Knot (ICK) Fold Inhibit Nicotinic Acetylcholine Receptors | en_US |
dc.type.version | publishedVersion | en_US |
dc.type | Journal article | en_US |
dc.type | Tidsskriftartikkel | en_US |
dc.type | Peer reviewed | en_US |