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dc.contributor.advisorWagner, Theresa
dc.contributor.advisorHegstad, Kristin
dc.contributor.authorEngi, Daniel
dc.date.accessioned2023-06-02T05:49:12Z
dc.date.available2023-06-02T05:49:12Z
dc.date.issued2021-06-01en
dc.description.abstractInvasive infection by multidrug-resistant Enterococcus faecium is increasingly becoming a healthcare concern. Few studies have addressed whether and how clinical E. faecium strains are able to outcompete commensal strains. This thesis aimed to characterize the in vitro competition between commensal and clinical E. faecium strains with respect to growth inhibition exerted by bacteriocins produced by the respective strain groups. A combination of laboratory assays and bioinformatical methods were applied toward this goal. Laboratory assays consisted of competitively growing commensal and clinical E. faecium strains, and using unmodified and modified supernatants from the same strains to investigate the nature of growth inhibition-mediating agents. The bioinformatical methods consisted of genetic relationship analyses and ribosomally synthesized post-translationally modified peptide-mining. It was shown that clinical E. faecium strains generally outcompete commensal E. faecium strains in vitro, though the commensal E. faecium strains that inhibit the growth of clinical E. faecium strains most often, do so very strongly. Furthermore, some commensal and clinical E. faecium strains possibly encoding novel bacteriocins were identified. Finally some commensal E. faecium strains that were resistant to the growth-inhibiting mechanisms of most epidemic and hospital-associated clinical E. faecium strains were found.en_US
dc.identifier.urihttps://hdl.handle.net/10037/29322
dc.language.isoengen_US
dc.publisherUiT Norges arktiske universitetno
dc.publisherUiT The Arctic University of Norwayen
dc.rights.holderCopyright 2021 The Author(s)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)en_US
dc.subject.courseIDMED-3950
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::Cell biology: 471en_US
dc.subjectEnterococcus faeciumen_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::General microbiology: 472en_US
dc.subjectEnterococcus faeciumen_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474en_US
dc.subjectPhylogeneticsen_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::Bioinformatics: 475en_US
dc.subjectRiPP miningen_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::Molecular biology: 473en_US
dc.subjectBacteriocinsen_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::General microbiology: 472en_US
dc.subjectCompetitive growthen_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::General microbiology: 472en_US
dc.subjectGrowth inhibitionen_US
dc.titleCharacterization of competition between commensal and clinical strains of Enterococcus faeciumen_US
dc.typeMaster thesisen
dc.typeMastergradsoppgaveno


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Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)