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dc.contributor.authorRichard, Celine Sarah Marine
dc.contributor.authorDey, Hymonti
dc.contributor.authorØyen, Frode Jacobsen
dc.contributor.authorMaqsood, Munazza
dc.contributor.authorBlencke, Hans-Matti
dc.date.accessioned2023-08-29T07:39:01Z
dc.date.available2023-08-29T07:39:01Z
dc.date.issued2023-02-07
dc.description.abstractReporter genes are important tools in many biological disciplines. The discovery of novel reporter genes is relatively rare. However, known reporter genes are constantly applied to novel applications. This study reports the performance of the bilirubin-dependent fluorescent protein UnaG from the Japanese eel Anguilla japonicas in live Escherichia coli cells in response to the disruption of outer membrane (OM) integrity at low bilirubin (BR) concentrations. Using the E. coli wild-type strain MC4100, its isogenic OM-deficient mutant strain NR698, and different OM-active compounds, we show that BR uptake and UnaG fluorescence depend on a leaky OM at concentrations of 10 µM BR and below, while fluorescence is mostly OM integrity-independent at concentrations above 50 µM BR. We suggest that these properties of the UnaG–BR couple might be applied as a biosensor as an alternative to the OM integrity assays currently in use.en_US
dc.identifier.citationRichard CSM, Dey H, Øyen F, Maqsood, Blencke H. Outer Membrane Integrity-Dependent Fluorescence of the Japanese Eel UnaG Protein in Live Escherichia coli Cells. Biosensors. 2023;13(2)en_US
dc.identifier.cristinIDFRIDAID 2123605
dc.identifier.doi10.3390/bios13020232
dc.identifier.issn2079-6374
dc.identifier.urihttps://hdl.handle.net/10037/30492
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.relation.ispartofDey, H. (2024). Antimicrobial activities and mechanisms of action of peptide analogues of marine origin. (Doctoral thesis). <a href=https://hdl.handle.net/10037/33605>https://hdl.handle.net/10037/33605</a>
dc.relation.ispartofRichard, C.S.M. (2024). Engineering novel bacterial biosensors for the characterization of membrane active natural products. (Doctoral thesis). <a href=https://hdl.handle.net/10037/33610>https://hdl.handle.net/10037/33610</a>
dc.relation.journalBiosensors
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2023 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleOuter Membrane Integrity-Dependent Fluorescence of the Japanese Eel UnaG Protein in Live Escherichia coli Cellsen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Med mindre det står noe annet, er denne innførselens lisens beskrevet som Attribution 4.0 International (CC BY 4.0)