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dc.contributor.authorBariås, Espen
dc.contributor.authorJakubec, Martin
dc.contributor.authorFørsund, Elise
dc.contributor.authorHjørnevik, Linda Veka
dc.contributor.authorLewis, Aurelia Eva
dc.contributor.authorHalskau, Øyvind
dc.date.accessioned2023-08-30T11:49:54Z
dc.date.available2023-08-30T11:49:54Z
dc.date.issued2023-03-14
dc.description.abstractLipids have been implicated in Parkinson's Disease (PD). We therefore studied the lipid profile of the neuroblastoma SH-SY5Y cell line, which is used extensively in PD research and compared it to that of the A431 epithelial cancer cell line. We have isolated whole cell extracts (WC) and plasma membrane (PM) fractions of both cell lines. The isolates were analyzed with 31P NMR. We observed a significant higher abundance of phosphatidylcholine (PC) for SH-SY5Y cells for both WC (55 ± 4.1%) and PM (63.3 ± 3.1%) compared to WC (40.5 ± 2.2%) and PM (43.4 ± 1.3%) of A431. Moreover, a higher abundance of phosphatidylethanolamine was detected for the WC of A431 compared to the SH-SY5Y. Using LC-MS/MS, we also determined the relative abundance of fatty acid (FA) moieties for each phospholipid class, finding that SH-SY5Y had high polyunsaturated FA levels, including arachidonic acid compared to A431 cells. When comparing our results to reported compositions of brain and neural tissues, we note the much higher PC levels, as well as very low levels of docosahexaenoic acid. However, relative levels of arachidonic acid and other polyunsaturated fatty acids were elevated, in line with what is desirable for a neural model system.en_US
dc.identifier.citationBariås E, Jakubec M, Førsund E, Hjørnevik LV, Lewis AE, Halskau ØH. Contrasting the phospholipid profiles of two neoplastic cell lines reveal a high PC:PE ratio for SH-SY5Y cells relative to A431 cells. Biochemical and Biophysical Research Communications - BBRC. 2023;656:23-29en_US
dc.identifier.cristinIDFRIDAID 2145625
dc.identifier.doi10.1016/j.bbrc.2023.03.017
dc.identifier.issn0006-291X
dc.identifier.issn1090-2104
dc.identifier.urihttps://hdl.handle.net/10037/30556
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalBiochemical and Biophysical Research Communications - BBRC
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2023 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0en_US
dc.rightsAttribution-NonCommercial 4.0 International (CC BY-NC 4.0)en_US
dc.titleContrasting the phospholipid profiles of two neoplastic cell lines reveal a high PC:PE ratio for SH-SY5Y cells relative to A431 cellsen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
Med mindre det står noe annet, er denne innførselens lisens beskrevet som Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)