Show simple item record

dc.contributor.authorHamre, Anne Grethe
dc.contributor.authorAl-Sadawi, Rim
dc.contributor.authorJohannesen, Kirsti Merete
dc.contributor.authorBisarro, Bastien
dc.contributor.authorKjendseth, Åsmund Røhr
dc.contributor.authorLeiros, Hanna-Kirsti S.
dc.contributor.authorSørlie, Morten
dc.date.accessioned2023-11-22T13:19:58Z
dc.date.available2023-11-22T13:19:58Z
dc.date.issued2023-09-19
dc.description.abstractSuperoxide dismutases (SODs) are enzymes that catalyze the dismutation of the superoxide radical anion into O<sup>2</sup> and H<sup>2</sup>O<sup>2</sup> in a two-step reaction. They are ubiquitous to all forms of life and four different types of metal centers are detected, dividing this class of enzymes into Cu-/Zn-, Ni-, Mn-, and Fe-SODs. In this study, a superoxide dismutase from the thermophilic bacteria Thermobifda fusca (TfSOD) was cloned and expressed before the recombinant enzyme was characterized. The enzyme was found to be active for superoxide dismutation measured by inhibition of cytochrome c oxidation and the inhibition of the autoxidation of pyrogallol. Its pH-optimum was determined to be 7.5, while it has a broad temperature optimum ranging from 20 to 90 °C. Combined with the T<sub>m</sub> that was found to be 78.5±0.5 °C at pH 8.0, TfSOD can be defined as a thermostable enzyme. Moreover, the crystal structure of TfSOD was determined and refined to 1.25 Å resolution. With electron paramagnetic resonance spectroscopy, it was confirmed that iron is the metal co-factor of TfSOD. The cell potential (E<sub>m</sub>) for the TfSOD-Fe<sup>3+</sup>/TfSOD-Fe<sup>2+</sup> redox couple was determined to be 287 mV.en_US
dc.identifier.citationHamre, Al-Sadawi, Johannesen, Bisarro, Kjendseth, Leiros, Sørlie. Initial characterization of an iron superoxide dismutase from Thermobifida fusca. JBIC Journal of Biological Inorganic Chemistry. 2023en_US
dc.identifier.cristinIDFRIDAID 2181957
dc.identifier.doi10.1007/s00775-023-02019-9
dc.identifier.issn0949-8257
dc.identifier.issn1432-1327
dc.identifier.urihttps://hdl.handle.net/10037/31847
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.relation.journalJBIC Journal of Biological Inorganic Chemistry
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2023 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleInitial characterization of an iron superoxide dismutase from Thermobifida fuscaen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


File(s) in this item

Thumbnail

This item appears in the following collection(s)

Show simple item record

Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)