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dc.contributor.authorOdeberg, Lynn Butler
dc.contributor.authorStruck, Eike Christopher
dc.contributor.authorOdeberg, Jacob Olof
dc.date.accessioned2024-01-22T14:18:39Z
dc.date.available2024-01-22T14:18:39Z
dc.date.issued2024-11-29
dc.description.abstractThe vascular endothelium acts as a dynamic interface between blood and tissue. TNF-α, a major regulator of inflammation, induces endothelial cell (EC) transcriptional changes, the overall response dynamics of which have not been fully elucidated. In the present study, we conducted an extended time-course analysis of the human EC response to TNF, from 30 min to 72 h. We identified regulated genes and used weighted gene network correlation analysis to decipher coexpression profiles, uncovering two distinct temporal phases: an acute response (between 1 and 4 h) and a later phase (between 12 and 24 h). Sex-based subset analysis revealed that the response was comparable between female and male cells. Several previously uncharacterized genes were strongly regulated during the acute phase, whereas the majority in the later phase were IFN-stimulated genes. A lack of IFN transcription indicated that this IFN-stimulated gene expression was independent of de novo IFN production. We also observed two groups of genes whose transcription was inhibited by TNF: those that resolved toward baseline levels and those that did not. Our study provides insights into the global dynamics of the EC transcriptional response to TNF, highlighting distinct gene expression patterns during the acute and later phases. Data for all coding and noncoding genes is provided on the Web site (http://www.endothelial-response.org/). These findings may be useful in understanding the role of ECs in inflammation and in developing TNF signaling–targeted therapies.en_US
dc.identifier.citationOdeberg, Struck, Odeberg. Global Transcriptome Analysis Reveals Distinct Phases of the Endothelial Response to TNF. Journal of Immunology. 2024en_US
dc.identifier.cristinIDFRIDAID 2220830
dc.identifier.doi10.4049/jimmunol.2300419
dc.identifier.issn0022-1767
dc.identifier.issn1550-6606
dc.identifier.urihttps://hdl.handle.net/10037/32679
dc.language.isoengen_US
dc.publisherThe American Association of Immunologists, Incen_US
dc.relation.journalJournal of Immunology
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2024 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleGlobal Transcriptome Analysis Reveals Distinct Phases of the Endothelial Response to TNFen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Med mindre det står noe annet, er denne innførselens lisens beskrevet som Attribution 4.0 International (CC BY 4.0)