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dc.contributor.authorLi, Xinyu
dc.contributor.authorChi, Heng
dc.contributor.authorDalmo, Roy Ambli
dc.contributor.authorTang, Xiaoqian
dc.contributor.authorXing, Jing
dc.contributor.authorSheng, Xiuzhen
dc.contributor.authorZhan, Wenbin
dc.date.accessioned2024-02-27T09:32:35Z
dc.date.available2024-02-27T09:32:35Z
dc.date.issued2023-10-21
dc.description.abstractInfections due to pathogens impact global aquaculture economy, where diseases caused by bacteria should be in particular focus due to antibiotic resistance. Hepcidin and NK-lysin are important innate immune factors having potential to be exploited as alternatives to antibiotics due to their antimicrobial activity and immunomodulatory capacity. In this study, the recombinant proteins of hepcidin 2 and NK-lysin (rPoHep2 and rPoNKL) from flounder (Paralichthys olivaceus) were obtained via a prokaryotic expression system. The results exhibited that rPoHep2 and rPoNKL killed both gram-negative and gram-positive bacteria mainly via attachment and disruption of the membrane. Interestingly, both peptides could bind to bacterial DNA. The antiviral assay showed that both peptides have antiviral activity against hirame nonvirhabdovirus. They exhibited no cytotoxicity to the mammalian and fish cell lines. PoHep2 was found localized in G-CSFR-positive peritoneal cells. Moreover, rPoHep2 significantly enhanced the phagocytosis of flounder leukocytes in vitro. These findings suggested that neutrophils contained rPoHep2 and may respond to the immunoreaction of neutrophils. In summary, both rPoHep2 and rPoNKL possess antimicrobial activities and may be exploited to replace traditional antibiotics. rPoHep2 possess immune regulatory functions, that can be further investigated as an immunostimulant in aquaculture.en_US
dc.identifier.citationLi, Chi, Dalmo, Tang, Xing, Sheng, Zhan. Anti-microbial activity and immunomodulation of recombinant hepcidin 2 and NK-lysin from flounder (Paralichthys olivaceus). International Journal of Biological Macromolecules. 2023;253en_US
dc.identifier.cristinIDFRIDAID 2196308
dc.identifier.doi10.1016/j.ijbiomac.2023.127590
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.urihttps://hdl.handle.net/10037/33048
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalInternational Journal of Biological Macromolecules
dc.relation.projectIDNorges forskningsråd: 301401en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2023 The Author(s)en_US
dc.subjectVDP::Medisinske fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.subjectVDP::Midical sciences: 700::Basic medical, dental and veterinary sciences: 710en_US
dc.titleAnti-microbial activity and immunomodulation of recombinant hepcidin 2 and NK-lysin from flounder (Paralichthys olivaceus)en_US
dc.type.versionacceptedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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