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dc.contributor.authorSalvo, Nina Mjølsnes
dc.contributor.authorOlsen, Gunn-Hege
dc.contributor.authorBerg, Thomas
dc.contributor.authorJanssen, Kirstin
dc.date.accessioned2024-06-14T07:30:00Z
dc.date.available2024-06-14T07:30:00Z
dc.date.issued2024-04-18
dc.description.abstractThe inference of biogeographical ancestry (BGA) can assist in police investigations of serious crime cases and help to identify missing people and victims of mass disasters. In this study, we evaluated the typing performance of 56 ancestry-informative SNPs in 177 samples using the ForenSeq™ DNA Signature Prep Kit on the MiSeq FGx system. Furthermore, we compared the prediction accuracy of the tools Universal Analysis Software v1.2 (UAS), the FROG-kb, and GenoGeographer when inferring the ancestry of 503 Europeans, 22 non-Europeans, and 5 individuals with co-ancestry. The kit was highly sensitive with complete aiSNP profiles in samples with as low as 250pg input DNA. However, in line with others, we observed low read depth and occasional drop-out in some SNPs. Therefore, we suggest not using less than the recommended 1ng of input DNA. FROG-kb and GenoGeographer accurately predicted both Europeans (99.6% and 91.8% correct, respectively) and non-Europeans (95.4% and 90.9% correct, respectively). The UAS was highly accurate when predicting Europeans (96.0% correct) but performed poorer when predicting non-Europeans (40.9% correct). None of the tools were able to correctly predict individuals with co-ancestry. Our study demonstrates that the use of multiple prediction tools will increase the prediction accuracy of BGA inference in forensic casework.en_US
dc.identifier.citationSalvo, Olsen, Berg, Janssen. Biogeographical Ancestry Analyses Using the ForenSeq<sup>TM</sup> DNA Signature Prep Kit and Multiple Prediction Tools. Genes. 2024;15(4)en_US
dc.identifier.cristinIDFRIDAID 2268125
dc.identifier.doi10.3390/genes15040510
dc.identifier.issn2073-4425
dc.identifier.urihttps://hdl.handle.net/10037/33806
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.relation.ispartofSalvo, N.M. (2024). Forensic DNA phenotyping - Towards reliable and accurate DNA tests for prediction of eye colour, hair colour and biogeographical ancestry. (Doctoral thesis). <a href=https://hdl.handle.net/10037/33807>https://hdl.handle.net/10037/33807</a>
dc.relation.journalGenes
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2024 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleBiogeographical Ancestry Analyses Using the ForenSeq™ DNA Signature Prep Kit and Multiple Prediction Toolsen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)