Institutt for medisinsk biologi: Nye registreringer
Viser treff 321-340 av 1154
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Prognostic value of absolute quantification of mutated KRAS in circulating tumour DNA in lung adenocarcinoma patients prior to therapy
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-01-27)Droplet digital polymerase chain reaction (ddPCR) is a highly sensitive and accurate method for quantification of nucleic acid sequences. We used absolute quantification of mutated v‐Ki‐ras2 Kirsten rat sarcoma viral oncogene homology gene (KRAS) by ddPCR to investigate the prognostic role of mutated KRAS in patients with KRAS‐mutated lung adenocarcinomas. Pre‐treatment plasma samples from 60 patients ... -
Tumor-associated macrophages and macrophage-related immune checkpoint expression in sarcomas
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-04-12)Early trials for immune checkpoint inhibitors in sarcomas have delivered mixed results, and efforts to improve outcomes now look to combinatorial strategies with novel immunotherapeutics, including some that target macrophages. To enhance our understanding of the sarcoma immune landscape, we quantified and characterized tumor-associated macrophage infiltration and expression of the targetable ... -
Exploring Conformational Dynamics of the Extracellular Venus flytrap Domain of the GABA B Receptor: A Path-Metadynamics Study
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-04-01)γ-Aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the central nervous system (CNS). Dysfunctional GABAergic neurotransmission is associated with numerous neurological and neuropsychiatric disorders. The GABA<sub>B</sub> receptor (GABA<sub>B</sub>-R) is a heterodimeric class C G protein-coupled receptor (GPCR) comprised of GABA<sub>B1a/b</sub> and GABA<sub>B2</sub> subunits. The ... -
Kidney tertiary lymphoid structures in Lupus Nephritis develop into large interconnected networks and resemble lymph nodes in gene signature
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-08-16)Immune aggregates organized as tertiary lymphoid structures (TLS) are observed within the kidneys of patients with systemic lupus erythematosus and lupus nephritis (LN). Renal TLS was characterized in lupus-prone New Zealand black × New Zealand white F1 mice analyzing cell composition and vessel formation. RNA sequencing was performed on transcriptomes isolated from lymph nodes, macrodissected TLS ... -
Medisinsk tannturisme: Kan behandling utenfor Norden medføre økt risiko for bærerskap og infeksjoner av antibiotika resistente bakterier?
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-01-03)I de senere år har antibiotikaresistente bakterier blitt et økende medisinsk problem. Slike bakterier har smittet medisinske turister, og blitt bragt hjem til Norden. Det er beregnet at det i verden er nærmere 20 millioner pasienter som reiser internasjonalt, og at kostnadene er over 50 milliarder US dollar. I Europa er det pasienter som ønsker billig tannbehandling som utgjør hovedmengden av ... -
Genetic Diversity of the Noncoding Control Region of the Novel Human Polyomaviruses
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-12-07)The genomes of polyomaviruses are characterized by their tripartite organization with an early region, a late region and a noncoding control region (NCCR). The early region encodes proteins involved in replication and transcription of the viral genome, while expression of the late region generates the capsid proteins. Transcription regulatory sequences for expression of the early and late genes, as ... -
Identification and experimental confirmation of novel cGMP efflux inhibitors by virtual ligand screening of vardenafil-analogues
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-03-28)<i>Background</i> - Clinical studies have reported overexpression of PDE5 and elevation of intracellular cyclic GMP in various types of cancer cells. ABCC5 transports cGMP out of the cells with high affinity. PDE5 inhibitors prevent both cellular metabolism and cGMP efflux by inhibiting ABCC5 as well as PDE5. Increasing intracellular cGMP is hypothesized to promote apoptosis and growth restriction ... -
CALCOCO1 acts with VAMP ‐associated proteins to mediate ER ‐phagy
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-06-11)The endoplasmic reticulum (ER) plays important roles in protein synthesis and folding, and calcium storage. The volume of the ER and expression of its resident proteins are increased in response to nutrient stress. ER‐phagy, a selective form of autophagy, is involved in the degradation of the excess components of the ER to restore homeostasis. Six ER‐resident proteins have been identified as ER‐phagy ... -
FuncPEP: A Database of Functional Peptides Encoded by Non-Coding RNAs
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-09-23)Non-coding RNAs (ncRNAs) are essential players in many cellular processes, from normal development to oncogenic transformation. Initially, ncRNAs were defined as transcripts that lacked an open reading frame (ORF). However, multiple lines of evidence suggest that certain ncRNAs encode small peptides of less than 100 amino acids. The sequences encoding these peptides are known as small open reading ... -
A Holistic Perspective: Exosomes Shuttle between Nerves and Immune Cells in the Tumor Microenvironment
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-10-31)One of the limitations of cancer research has been the restricted focus on tumor cells and the omission of other non-malignant cells that are constitutive elements of this systemic disease. Current research is focused on the bidirectional communication between tumor cells and other components of the tumor microenvironment (TME), such as immune and endothelial cells, and nerves. A major success of ... -
Current Status of Circulating Tumor Cells, Circulating Tumor DNA, and Exosomes in Breast Cancer Liquid Biopsies
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-12-11)Breast cancer is the most common cancer among women worldwide. Although the five-, ten- and fifteen-year survival rates are good for breast cancer patients diagnosed with early-stage disease, some cancers recur many years after completion of primary therapy. Tumor heterogeneity and clonal evolution may lead to distant metastasis and therapy resistance, which are the main causes of breast cancer-associated ... -
Protein kinase C isozymes associated with relapse free survival in non-small cell lung cancer patients
(Journal article; Tidsskriftartikkel, 2020-11-25)<i>Introduction</i>: Protein expression is deregulated in cancer, and the proteomic changes observed in lung cancer may be a consequence of mutations in essential genes. The purpose of this study was to identify protein expression associated with prognosis in lung cancers stratified by smoking status, molecular subtypes, and EGFR-, TP53-, and KRAS-mutations.<p> <p><i>Methods</i>: We performed ... -
NADPH oxidase 2 mediates myocardial oxygen wasting in obesity
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-02-19)Obesity and diabetes are independent risk factors for cardiovascular diseases, and they are associated with the development of a specific cardiomyopathy with elevated myocardial oxygen consumption (MVO<sub>2</sub>) and impaired cardiac efficiency. Although the pathophysiology of this cardiomyopathy is multifactorial and complex, reactive oxygen species (ROS) may play an important role. One of the ... -
Monkeypox Virus in Nigeria: Infection Biology, Epidemiology, and Evolution
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-11-05)Monkeypox is a zoonotic disease caused by monkeypox virus (MPXV), which is a member of orthopoxvirus genus. The reemergence of MPXV in 2017 (at Bayelsa state) after 39 years of no reported case in Nigeria, and the export of travelers’ monkeypox (MPX) from Nigeria to other parts of the world, in 2018 and 2019, respectively, have raised concern that MPXV may have emerged to occupy the ecological and ... -
Data on RT-qPCR assay of nuclear progesterone receptors (nPR), membrane progesterone receptors (mPR) and progesterone receptor membrane components (PGRMC) from human uterine endometrial tissue and cancer cells of the uterine cervix
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-06-25)A previous investigation showed that the endometrium normalized in women with endometrial hyperplasia after three months treatment with high dose levonorgestrel IUS (intrauterine system). The effect was maintained even if immunohistochemical analyses of the endometrium showed that nuclear progesterone receptors (nPRs) were completely downregulated. These observations indicated that some type of ... -
Bicaudal D1 impairs autophagosome maturation in chronic obstructive pulmonary disease
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-09-26)Bicaudal D1 (BICD1), an adaptor for the dynein‐dynactin motor complex, has been identified as a susceptibility gene in chronic obstructive pulmonary disease (COPD). Autophagy, an essential cellular homeostasis process, is defective in COPD, in which oxidative stress‐induced misfolded proteins accumulate into toxic aggregates dependent on the accumulation of the autophagic cargo receptor p62. Defective ... -
From Differential Stains to Next Generation Physiology: Chemical Probes to Visualize Bacterial Cell Structure and Physiology
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-10-26)Chemical probes have been instrumental in microbiology since its birth as a discipline in the 19th century when chemical dyes were used to visualize structural features of bacterial cells for the first time. In this review article we will illustrate the evolving design of chemical probes in modern chemical biology and their diverse applications in bacterial imaging and phenotypic analysis. We will ... -
Nox4 regulates InsP3 receptor‐dependent Ca2+ release into mitochondria to promote cell survival
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-08-10)Cells subjected to environmental stresses undergo regulated cell death (RCD) when homeostatic programs fail to maintain viability. A major mechanism of RCD is the excessive calcium loading of mitochondria and consequent triggering of the mitochondrial permeability transition (mPT), which is especially important in post‐mitotic cells such as cardiomyocytes and neurons. Here, we show that stress‐induced ... -
The Effects of Metformin on Age-Related Changes in the Liver Sinusoidal Endothelial Cell
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-06-14)Age-related changes in the liver sinusoidal endothelium, particularly the reduction in fenestrations, contribute to insulin resistance in old age. Metformin impacts on the aging process and improves insulin resistance. Therefore, the effects of metformin on the liver sinusoidal endothelium were studied. Metformin increased fenestrations in liver sinusoidal endothelial cells isolated from both young ... -
Olaparib monotherapy as primary treatment in unselected triple negative breast cancer
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-11-23)<p><i>Background - </i>The antitumor efficacy of PARP inhibitors (PARPi) for breast cancer patients harboring germline BRCA1/2 (gBRCA1/2) mutations is well established. While PARPi monotherapy was ineffective in patients with metastatic triple negative breast cancer (TNBC) wild type for BRCA1/2, we hypothesized that PARPi may be effective in primary TNBCs without previous chemotherapy exposure. < ...