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dc.contributor.authorTansirichaiya, Supathep
dc.contributor.authorSongsomboon, Kittikun
dc.contributor.authorChaianant, Nichamon
dc.contributor.authorLertsivawinyu, Wasawat
dc.contributor.authorAl-Haroni, Mohammed Hasan Hussein
dc.date.accessioned2024-09-19T08:46:14Z
dc.date.available2024-09-19T08:46:14Z
dc.date.issued2024-06-28
dc.description.abstractObjectives: The human oral microbiome, a complex ecosystem linked to oral and systemic health, harbors a diverse array of microbial populations, including antimicrobial resistance genes (ARGs). As a critical component of the One Health approach to tackle antibiotic resistance, comprehending the oral resistome's composition and diversity is imperative. The objective of this study was to investigate the impact of chemical cell lysis treatment using MetaPolyzyme on the detectability of the oral microbiome, resistome, and DNA quality and quantity.<p> <p>Materials and Methods: Saliva samples were collected from five healthy individuals, and each of the samples was subjected to DNA extraction with and without the treatment with MetaPolyzyme. Through metagenomic sequencing, we analyzed, assessed, and compared the microbial composition, resistome, and DNA characteristics between both groups of extracted DNA. <p>Results: Our study revealed that MetaPolyzyme treatment led to significant shifts in the detectability of microbial composition, favoring Gram‐positive bacteria, notably Streptococcus, over Gram‐negative counterparts. Moreover, the MetaPolyzyme treatment also resulted in a distinct change in ARG distribution. This shift was characterized by an elevated proportion of ARGs linked to fluoroquinolones and efflux pumps, coupled with a reduction in the prevalence of tetracycline and β‐lactam resistance genes when compared with the nontreated group. Alpha diversity analysis demonstrated altered species and ARG distribution without affecting overall diversity, while beta diversity analysis confirmed significant differences in the taxonomical composition and oral resistome between treated and nontreated groups. <p>Conclusions: These findings underscore the critical role of cell lysis treatment in optimizing oral metagenomic studies and enhance our understanding of the oral resistome's dynamics in the context of antimicrobial resistance.en_US
dc.identifier.citationTansirichaiya S, Songsomboon, Chaianant, Lertsivawinyu, Al-Haroni M. Impact of cell lysis treatment before saliva metagenomic DNA extraction on the oral microbiome and the associated resistome. Clinical and Experimental Dental Research. 2024;10(4)en_US
dc.identifier.cristinIDFRIDAID 2281472
dc.identifier.doi10.1002/cre2.905
dc.identifier.issn2057-4347
dc.identifier.urihttps://hdl.handle.net/10037/34793
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.journalClinical and Experimental Dental Research
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2024 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleImpact of cell lysis treatment before saliva metagenomic DNA extraction on the oral microbiome and the associated resistomeen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)