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dc.contributor.authorHenriksen, Jørn Remi
dc.contributor.authorHaug, Bjørn Helge
dc.contributor.authorBüchner, Jochen
dc.contributor.authorTømte, Ellen
dc.contributor.authorLØkke, cecilie
dc.contributor.authorFlaegstad, Trond
dc.contributor.authorEinvik, christer
dc.date.accessioned2011-10-10T10:24:30Z
dc.date.available2011-10-10T10:24:30Z
dc.date.issued2011
dc.description.abstractBackground: Neuroblastoma is a childhood cancer derived from immature cells of the sympathetic nervous system. The disease is clinically heterogeneous, ranging from neuronal differentiated benign ganglioneuromas to aggressive metastatic tumours with poor prognosis. Amplification of the MYCN oncogene is a well established poor prognostic factor found in up to 40% of high risk neuroblastomas. Using neuroblastoma cell lines to study neuronal differentiation in vitro is now well established. Several protocols, including exposure to various agents and growth factors, will differentiate neuroblastoma cell lines into neuron-like cells. These cells are characterized by a neuronal morphology with long extensively branched neurites and expression of several neurospecific markers. <br>Results: In this study we use retrovirally delivered inducible short-hairpin RNA (shRNA) modules to knock down MYCN expression in MYCN-amplified (MNA) neuroblastoma cell lines. By addition of the inducer doxycycline, we show that the Kelly and SK-N-BE(2) neuroblastoma cell lines efficiently differentiate into neuron-like cells with an extensive network of neurites. These cells are further characterized by increased expression of the neuronal differentiation markers NFL and GAP43. In addition, we show that induced expression of retrovirally delivered anti- MYCN shRNA inhibits cell proliferation by increasing the fraction of MNA neuroblastoma cells in the G1 phase of the cell cycle and that the clonogenic growth potential of these cells was also dramatically reduced. <br>Conclusion: We have developed an efficient MYCN-knockdown in vitro model system to study neuronal differentiation in MNA neuroblastomas.en
dc.identifier.citationBMC Developmental Biology (2011), 11:1en
dc.identifier.cristinIDFRIDAID 517123
dc.identifier.issn1471-213X
dc.identifier.urihttps://hdl.handle.net/10037/3642
dc.identifier.urnURN:NBN:no-uit_munin_3358
dc.language.isoengen
dc.publisherBioMed Centralen
dc.rights.accessRightsopenAccess
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750::Oncology: 762en
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Onkologi: 762en
dc.titleConditional expression of retrovirally delivered anti-MYCN shRNA as an in vitro model system to study neuronal differentiation in MYCN-amplified neuroblastomaen
dc.typeJournal articleen
dc.typeTidsskriftartikkelen
dc.typePeer revieweden


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