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dc.contributor.authorAndrade, Rafaella Silva
dc.contributor.authorOliveira, Marina Martins de
dc.contributor.authorBueno Filho, Júlio Sílvio de Sousa
dc.contributor.authorFerreira, Fernando
dc.contributor.authorGodfroid, Jacques Xavier Leon
dc.contributor.authorLage, Andrey Pereira
dc.contributor.authorDorneles, Elaine Maria Seles
dc.date.accessioned2025-02-07T13:37:17Z
dc.date.available2025-02-07T13:37:17Z
dc.date.issued2023-11-21
dc.description.abstractThe direct methods for diagnosis of bovine brucellosis have several limitations, therefore serological tests are the basis for the diagnosis of the disease. However, a meta-analysis estimating the diagnostic sensitivity (DSe) and diagnostic specificity (DSp) on the main tests used in bovine brucellosis control programs worldwide has not been performed. This systematic review and meta-analysis aimed to estimate the DSe, DSp and thereby accuracy of serological tests individually used in the diagnosis of bovine brucellosis. The databases CABI, Cochrane Library, PubMed/MEDLINE, SciELO, Scopus and Web of Science were used to select articles. The search resulted in 5308 studies, of which 71 were selected for systematic review using quality assessment tools and 65 studies were included in the meta-analysis. For the meta-analysis, 178 assays and 11 different serological tests were considered. To estimate DSe and DSp of the tests, studies were divided according to animal selection for the studies: (1) studies that carried out a random or consecutive selection of participants (noncasesingle bondcontrol studies) and (2) all studies, including casesingle bondcontrol studies. Considering only the non-case-control studies to estimate the DSe, the tests that exhibited the best and worst performance were the iELISA test (indirect enzyme immunoassay - bacterial suspension as antigen - BS) (96.5%, 95% CI: 94.1–97.9%) and 2ME (2- mercaptoethanol test) (85.0%, 95% CI: 79.6–89.1%), respectively; while for DSp, the FPA (fluorescence polarization assay) (99, 7%, 95% CI: 99.5–99.8%) and PCFIA tests (protein concentration fluorescence immunoassay) (78.5%, 95% CI: 70.0–85.1%) showed better and worse performance, respectively. Overall, our results showed an overestimation in the DSe and DSp of the eleven serological tests assessed when casesingle bondcontrol studies were included in the meta-analysis, which is a concern considering its impacts on the time and costs associated with populational diagnosis of the diseases, since several of these tests are routinely used in the control and eradication programs of bovine brucellosis worldwide. Furthermore, the tests that exhibited the best DSe and DSp, iELISA (BS) and FPA, respectively, are relatively easy to perform and interpret and the test which showed the best overall accuracy was FPA.en_US
dc.identifier.citationAndrade, Oliveira, Bueno Filho, Ferreira, Godfroid, Lage, Dorneles. Accuracy of serological tests for bovine brucellosis: A systematic review and meta-analysis. Preventive Veterinary Medicine. 2024;222en_US
dc.identifier.cristinIDFRIDAID 2236850
dc.identifier.doi10.1016/j.prevetmed.2023.106079
dc.identifier.issn0167-5877
dc.identifier.issn1873-1716
dc.identifier.urihttps://hdl.handle.net/10037/36434
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalPreventive Veterinary Medicine
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2024 The Author(s)en_US
dc.titleAccuracy of serological tests for bovine brucellosis: A systematic review and meta-analysisen_US
dc.type.versionacceptedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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