Blar i forfatter Det helsevitenskapelige fakultet "Øie, Cristina Ionica"

Viser treff 1-16 av 16

    • Asialoglycoprotein Receptor (ASGP-R) is Liver Parenchymal Cells is Involved in Elimination of Recombinant Human TFPI 

      Appa, Rupa Shree; Øie, Cristina Ionica; Brodin, Ellen; Hansen, John-Bjarne; Smedsrød, Bård (Journal article; Tidsskriftartikkel, 2010)
      We here report on a study carried out to determine the early clearance kinetics, and organ, cell(s) and receptor(s) responsible for the clearance of full length TFPI purified from BHK cells (TFPI<sup>BHK</sup>). Following intravenous administration, <sup>125</sup>I-TFPI<sup>BHK</sup> was cleared with a biphasic elimination curve, and with a significantly slower t<sub>1/2</sub>α compared to recombinant ...

    • Effect of Unfractionated Heparin on TFPI Elimination 

      Øie, Cristina Ionica; Appa, Rupa Shree; Brodin, Ellen; Hilden, Ida; Smedsrød, Bård; Hansen, John-Bjarne (Journal article; Tidsskriftartikkel, 2010)
      Background: Tissue factor pathway inhibitor (TFPI) plays an important role for the anticoagulant effect of heparin. Depletion of intravascular TFPI by treatment with unfractionated heparin (UFH), and not by low molecular weight heparin (LMWH), has been suggested to explain the superiority of LMWH in treatment of both arterial and venous thrombosis. The present study was undertaken to investigate ...

    • FITC conjugation markedly enhances hepatic clearance of N-formyl peptides 

      Øie, Cristina Ionica; Snapkov, Igor; Elvevold, Kjetil; Sveinbjørnsson, Baldur; Smedsrød, Bård (Journal article; Tidsskriftartikkel; Peer reviewed, 2016-08-05)
      In both septic and aseptic inflammation, N-formyl peptides may enter the circulation and induce a systemic inflammatory response syndrome similar to that observed during septic shock. The inflammatory response is brought about by the binding of N-formyl peptide to formyl peptide receptors (FPRs), specific signaling receptors expressed on myeloid as well as non-myeloid cells involved in the ...

    • Genome-wide analysis of DNA methylation and gene Expression patterns in purified, uncultures human liver cells and activated hepatic stellate cells 

      Taghdouini, Adil El; Sørensen, Anita Løvstad; Reiner, Andrew Henry; Coll, Mar; Verhulst, Stefaan; Mannaerts, Inge; Øie, Cristina Ionica; Smedsrød, Bård; Najimi, Mustapha; Sokal, Etienne; Luttun, Aernout; Sancho-Bru, Pau; Collas, Philippe; Grunsven, Leo A van (Journal article; Tidsskriftartikkel; Peer reviewed, 2015-08-31)
      <p>Background & Aims: Liver fibrogenesis – scarring of the liver that can lead to cirrhosis and liver cancer – is characterized by hepatocyte impairment, capillarization of liver sinusoidal endothelial cells (LSECs) and hepatic stellate cell (HSC) activation. To date, the molecular determinants of a healthy human liver cell phenotype remain largely uncharacterized. Here, we assess the transcriptome ...

    • High-affinity von Willebrand factor binding does not affect the anatomical or hepatocellular distribution of factor VIII in rats 

      Øie, Cristina Ionica; Roepstorff, Kirstine; Behrens, Carsten; Bøggild Kristensen, Jesper; Karpf, Ditte Maria; Bolt, Gert; Gudme, Charlotte N.; Kjalke, Marianne; Smedsrød, Bård; Appa, Rupa Shree (Journal article; Tidsskriftartikkel; Peer reviewed, 2016-07-05)
      Background: Von Willebrand factor (VWF) stabilizes factor VIII in the circulation and prevents its premature clearance.<p><p> Objective: To study the effects of VWF on FVIII clearance in rats with endogenous VWF.<p><p> Methods: Anatomical and hepatocellular distribution studies were performed in rats following intravenous administration of glycoiodinated recombinant FVIII (rFVIII) and a ...

    • In vitro generation of functional liver organoid-like structures using adult human cells 

      Ramachandran, Sarada Devi; Schirmer, Katharina; Münst, Bernhard; Heinz, Stefan; Ghafoory, Shahrouz; Wölfl, Stefan; Simon-Keller, Katja; Marx, Alexander; Øie, Cristina Ionica; Ebert, Matthias P.; Walles, Heike; Braspenning, Joris; Breitkopf-Heinlein, Katja (Journal article; Tidsskriftartikkel; Peer reviewed, 2015-10-21)
      In this study we used differentiated adult human upcyte1 cells for the in vitro generation of liver organoids. Upcyte1 cells are genetically engineered cell strains derived from primary human cells by lenti-viral transduction of genes or gene combinations inducing transient proliferation capacity (upcyte1 process). Proliferating upcyte1 cells undergo a finite number of cell divisions, i.e., ...

    • The influence of oxygen tension on the structure and function of isolated liver sinusoidal endothelial cells 

      Martinez, Inigo Zubiavrre; Warren, A; Le Couteur, D.G.; Nedredal, Geir Ivar; Øie, Cristina Ionica; Johansen, oddmund; Smedsrød, Bård (Journal article; Tidsskriftartikkel; Peer reviewed, 2008)

    • Liver sinusoidal endothelial cells contribute to the uptake and degradation of entero bacterial viruses 

      Øie, Cristina Ionica; Wolfson, Deanna; Yasunori, Tanji; Dumitriu, Gianina; Sørensen, Karen Kristine; McCourt, Peter Anthony; Ahluwalia, Balpreet Singh; Smedsrød, Bård (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-01-21)
      The liver is constantly exposed to dietary antigens, viruses, and bacterial products with inflammatory potential. For decades cellular uptake of virus has been studied in connection with infection, while the few studies designed to look into clearance mechanisms focused mainly on the role of macrophages. In recent years, attention has been directed towards the liver sinusoidal endothelial cells ...

    • Multimodal super-resolution optical microscopy visualizes the close connection between membrane and the cytoskeleton in liver sinusoidal endothelial cell fenestrations 

      Mönkemöller, Viola; Øie, Cristina Ionica; Hubner, Wolfgang; Huser, Thomas Rolf; McCourt, Peter Anthony (Journal article; Tidsskriftartikkel; Peer reviewed, 2015)
      Liver sinusoidal endothelial cells (LSECs) act as a filter between blood and the hepatocytes. LSECs are highly fenestrated cells; they contain transcellular pores with diameters between 50 to 200 nm. The small sizes of the fenestrae have so far prohibited any functional analysis with standard and advanced light microscopy techniques. Only the advent of super-resolution optical fluorescence microscopy ...

    • Multiscale and Multimodal Optical Imaging of the Ultrastructure of Human Liver Biopsies 

      Kong, Cihang; Bobe, Stefanie; Pilger, Christian; Lachetta, Mario; Øie, Cristina Ionica; Kirschnick, Nils; Mönkemöller, Viola; Hübner, Wolfgang; Förster, Christine; Schuttpelz, Mark; Kiefer, Friedemann; Huser, Thomas; Schulte am Esch, Jan (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-02-17)
      The liver as the largest organ in the human body is composed of a complex macroscopic and microscopic architecture that supports its indispensable function to maintain physiological homeostasis. Optical imaging of the human liver is particularly challenging because of the need to cover length scales across 7 orders of magnitude (from the centimeter scale to the nanometer scale) in order to fully ...

    • New insights into the clearance of tissue factor pathway inhibitor (TFPI) and unfractionated heparin (UFH) 

      Øie, Cristina Ionica (Doctoral thesis; Doktorgradsavhandling, 2010-11-05)
      ”Hvor mye?”, ”Hvor ofte?” og ”Hvor lenge?” er sentrale spørsmål i studier av legemidler. For å finne en optimal behandling trenger man kunnskap om et legemiddels opptak, fordeling i kroppen, og nedbryting. Øie sitt prosjekt viser at leveren spiller en nøkkelrolle i nedbrytningen av to sentrale substanser som hemmer blodlevring (antikoagulantia), heparin og tissue factor pathway inhibitor (TFPI). ...

    • Primary rat LSECs preserve their characteristic phenotype after cryopreservation 

      Mönkemöller, Viola; Mao, Hong; Hubner, Wolfgang; Heimann, Peter; Levy, Gahl; Huser, Thomas; Kaltschmidt, Barbara; Kaltschmidt, Christian; Øie, Cristina Ionica (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-10-02)
      Liver disease is a leading cause of morbidity and mortality worldwide. Recently, the liver non-parenchymal cells have gained increasing attention for their potential role in the development of liver disease. Liver sinusoidal endothelial cells (LSECs), a specialized type of endothelial cells that have unique morphology and function, play a fundamental role in maintaining liver homeostasis. Current ...

    • Quantitative analysis methods for studying fenestrations in liver sinusoidal endothelial cells. A comparative study 

      Szafranska, Karolina; Holte, Christopher Florian; Kruse, Larissa Dorothea; Mao, Hong; Øie, Cristina Ionica; Symonski, Marek; Zapotoczny, Bartlomiej; McCourt, Peter Anthony (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-07-28)
      Liver Sinusoidal Endothelial Cells (LSEC) line the hepatic vasculature providing blood filtration via transmembrane nanopores called fenestrations. These structures are 50−300 nm in diameter, which is below the resolution limit of a conventional light microscopy. To date, there is no standardized method of fenestration image analysis. With this study, we provide and compare three different approaches: ...

    • Quantitative analysis methods for studying fenestrations in liver sinusoidal endothelial cells. A comparative study 

      Szafranska, K.; Holte, Christopher Florian; Kruse, L.D.; Mao, Hong; Øie, Cristina Ionica; Szymonski, M.; Zapotoczny, B.; McCourt, P.A.G. (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-07-28)
      Liver Sinusoidal Endothelial Cells (LSEC) line the hepatic vasculature providing blood filtration via transmembrane nanopores called fenestrations. These structures are 50−300 nm in diameter, which is below the resolution limit of a conventional light microscopy. To date, there is no standardized method of fenestration image analysis. With this study, we provide and compare three different approaches: ...

    • Rat liver sinusoidal endothelial cells (LSECs) express functional low density lipoprotein receptor-related protein-1 (LRP-1) 

      Øie, Cristina Ionica; Appa, Rupa Shree; Hilden, Ida; Petersen, Helle Heibroch; Gruhler, Albrecht; Smedsrød, Bård; Hansen, John-Bjarne (Journal article; Tidsskriftartikkel; Peer reviewed, 2011)
      The low density lipoprotein receptor-related protein-1 (LRP-1) is a large, multifunctional endocytic receptor from the LDL receptor family, highly expressed in liver parenchymal cells (PCs), neurons, activated astrocytes, and fibroblasts. The aim of the study was to investigate if liver sinusoidal endothelial cells (LSECs), highly specialized scavenger cells, express LRP-1. To address this question, ...

    • SAMM50 acts with p62 in piecemeal basal- and OXPHOS-induced mitophagy of SAM and MICOS components 

      Abudu, Yakubu Princely; Shrestha, Birendra Kumar; Zhang, Wenxin; Palara, Anthimi; Brenne, Hanne Britt; Larsen, Kenneth Bowitz; Wolfson, Deanna; Dumitriu, Gianina; Øie, Cristina Ionica; Ahluwalia, Balpreet Singh; Levy, Gahl; Behrends, Christian; Tooze, Sharon A; Mouilleron, Stephane; Lamark, Trond; Johansen, Terje (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-05-26)
      Mitophagy is the degradation of surplus or damaged mitochondria by autophagy. In addition to programmed and stress-induced mitophagy, basal mitophagy processes exert organelle quality control. Here, we show that the sorting and assembly machinery (SAM) complex protein SAMM50 interacts directly with ATG8 family proteins and p62/SQSTM1 to act as a receptor for a basal mitophagy of components of the ...