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dc.contributor.authorHedberg, Annica
dc.contributor.authorKanapathippillai, Premasany
dc.contributor.authorRekvig, Ole Petter
dc.contributor.authorFenton, Kristin Andreassen
dc.date.accessioned2014-02-27T07:50:55Z
dc.date.available2014-02-27T07:50:55Z
dc.date.issued2013
dc.description.abstractWe have previously demonstrated that continuous infusion of low molecular weight (LMW) heparin delays autoantibody production and development of lupus nephritis in (NZBxNZW)F1 (B/W) mice. In this study we investigated the effect of LMW heparin on renal cytokine and chemokine expression and on nucleosome-mediated activation of nucleosome-specific splenocytes. Total mRNA extracted from kidneys of heparin-treated or -untreated B/W mice was analysed by qPCR for the expression of several cytokines, chemokines, and Toll-like receptors. Splenocytes taken from B/W mice were stimulated with nucleosomes with or without the presence of heparin. Splenocyte cell proliferation as thymidine incorporation and the expression of costimulatory molecules and cell activation markers were measured. Heparin treatment of B/W mice reduced the in vivo expression of CCR2, IL1𝛽�, and TLR7 compared to untreated B/W mice. Nucleosome-induced cell proliferation of splenocytes was not influenced by heparin. The expression of CD80, CD86, CD69, CD25, CTLA-4, and TLR 2, 7, 8, and 9 was upregulated upon stimulation by nucleosomes, irrespective of whether heparin was added to the cell culture or not. In conclusion, treatment with heparin lowers the kidney expression of proinflammatory mediators in B/W mice but does not affect nucleosomal activation of splenocytes.en
dc.identifier.citationClinical & Developmental Immunology (2013), Article ID 791262en
dc.identifier.cristinIDFRIDAID 1068386
dc.identifier.doihttp://dx.doi.org/10.1155/2013/791262
dc.identifier.issn1740-2522
dc.identifier.urihttps://hdl.handle.net/10037/5881
dc.identifier.urnURN:NBN:no-uit_munin_5574
dc.language.isoengen
dc.publisherHindawi Publishing Corporationen
dc.rights.accessRightsopenAccess
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical molecular biology: 711en
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk molekylærbiologi: 711en
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Physiopathology: 721en
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Patofysiologi: 721en
dc.titleLMW Heparin Prevents Increased Kidney Expression of Proinflammatory Mediators in (NZBxNZW)F1 Miceen
dc.typeJournal articleen
dc.typeTidsskriftartikkelen
dc.typePeer revieweden


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