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dc.contributor.authorTveit, Alexander Tøsdal
dc.contributor.authorTim, Urich
dc.contributor.authorSvenning, Mette Marianne
dc.date.accessioned2015-02-24T13:34:35Z
dc.date.available2015-02-24T13:34:35Z
dc.date.issued2014-07-11
dc.description.abstractRecent advances in meta-omics and particularly metatranscriptomic approaches have enabled detailed studies of the structure and function of microbial communities in many ecosystems. Molecular analyses of peat soils, ecosystems important to the global carbon balance, are still challenging due to the presence of coextracted substances that inhibit enzymes used in downstream applications. We sampled layers at different depths from two high-Arctic peat soils in Svalbard for metatranscriptome preparation. Here we show that enzyme inhibition in the preparation of metatranscriptomic libraries can be circumvented by linear amplification of diluted template RNA. A comparative analysis of mRNA-enriched and nonenriched metatranscriptomes showed that mRNA enrichment resulted in a 2-fold increase in the relative abundance of mRNA but biased the relative distribution of mRNA. The relative abundance of transcripts for cellulose degradation decreased with depth, while the transcripts for hemicellulose debranching increased, indicating that the polysaccharide composition of the peat was different in the deeper and older layers. Taxonomic annotation revealed that Actinobacteria and Bacteroidetes were the dominating polysaccharide decomposers. The relative abundances of 16S rRNA and mRNA transcripts of methanogenic Archaea increased substantially with depth. Acetoclastic methanogenesis was the dominating pathway, followed by methanogenesis from formate. The relative abundances of 16S rRNA and mRNA assigned to the methanotrophic Methylococcaceae, primarily Methylobacter, increased with depth. In conclusion, linear amplification of total RNA and deep sequencing constituted the preferred method for metatranscriptomic preparation to enable high-resolution functional and taxonomic analyses of the active microbiota in Arctic peat soil.en
dc.identifier.citationApplied and Environmental Microbiology 80(2014) nr. 18 s. 5761-5772en_US
dc.identifier.cristinIDFRIDAID 1174165
dc.identifier.doi10.1128/AEM.01030-14
dc.identifier.issn0099-2240
dc.identifier.urihttps://hdl.handle.net/10037/7183
dc.identifier.urnURN:NBN:no-uit_munin_6828
dc.language.isoengen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.rights.accessRightsopenAccess
dc.subjectVDP:472en_US
dc.titleMetatranscriptomic Analysis of Arctic Peat Soil Microbiotaen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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