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dc.contributor.authorBizuayehu, Teshome Tilahun
dc.contributor.authorJohansen, Steinar D
dc.contributor.authorPuvanendran, Velmurugu
dc.contributor.authorToften, Hilde
dc.contributor.authorBabiak, Igor
dc.date.accessioned2015-09-16T12:32:19Z
dc.date.available2015-09-16T12:32:19Z
dc.date.issued2015-04-17
dc.description.abstractBackground: Environmental temperature has serious implications in life cycle of aquatic ectotherms. Understanding the molecular mechanisms of temperature acclimation and adaptation of marine organisms is of the uttermost importance for ecology, fisheries, and aquaculture, as it allows modeling the effects of global warming on population dynamics. Regulatory molecules are major modulators of acclimation and adaptation; among them, microRNAs (miRNAs) are versatile and substantial contributors to regulatory networks of development and adaptive plasticity. However, their role in thermal plasticity is poorly known. We have asked whether the temperature and its shift during the early ontogeny (embryonic and larval development) affect the miRNA repertoire of Atlantic cod (Gadus morhua), and if thermal experience has long-term consequences in the miRNA profile. Results: We characterized miRNA during different developmental stages and in juvenile tissues using next generation sequencing. We identified 389 putative miRNA precursor loci, 120 novel precursor miRNAs, and 281 mature miRNAs. Some miRNAs showed stage- or tissue-enriched expression and miRNAs, such as the miR-17 ~ 92 cluster, myomiRs (miR-206), neuromiRs (miR-9, miR-124), miR-130b, and miR-430 showed differential expression in different temperature regimes. Long-term effect of embryonic incubation temperature was revealed on expression of some miRNAs in juvenile pituitary (miR-449), gonad (miR-27c, miR-30c, and miR-200a), and liver (let-7 h, miR-7a, miR-22, miR-34c, miR-132a, miR-192, miR-221, miR-451, miR-2188, and miR-7550), but not in brain. Some of differentially expressed miRNAs in the liver were confirmed using LNA-based rt-qPCR. The effect of temperature on methylation status of selected miRNA promoter regions was mostly inconclusive. Conclusions: Temperature elevation by several degrees during embryonic and larval developmental stages significantly alters the miRNA profile, both short-term and long-term. Our results suggest that a further rise in seas temperature might affect life history of Atlantic cod.en_US
dc.identifier.citationBMC Genomics (2015) 16:305en_US
dc.identifier.cristinIDFRIDAID 1238285
dc.identifier.doi10.1186/s12864-015-1503-7
dc.identifier.issn1471-2164
dc.identifier.urihttps://hdl.handle.net/10037/8079
dc.identifier.urnURN:NBN:no-uit_munin_7660
dc.language.isoengen_US
dc.rights.accessRightsopenAccess
dc.subjectAtlantic coden_US
dc.subjectEmbryonic developmenten_US
dc.subjectMethylationen_US
dc.subjectmiRNAen_US
dc.subjectThermal plasticityen_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480en_US
dc.titleTemperature during early development has long-term effects on microRNA expression in Atlantic coden_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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