dc.contributor.author | Löhr, Iren Høyland | |
dc.contributor.author | Hülter, Nils Fredrik | |
dc.contributor.author | Bernhoff, Eva | |
dc.contributor.author | Johnsen, Pål Jarle | |
dc.contributor.author | Sundsfjord, Arnfinn | |
dc.contributor.author | Naseer, Mohammed Umaer | |
dc.date.accessioned | 2016-03-04T11:14:26Z | |
dc.date.available | 2016-03-04T11:14:26Z | |
dc.date.issued | 2015-03-04 | |
dc.description.abstract | Objectives
To characterize the CTX-M-15-encoding plasmid in a Klebsiella pneumoniae ST17 strain,
responsible for an outbreak at a Norwegian neonatal intensive care unit and subsequent
colonization of affected children for up to two years. To identify plasmid-mediated features
relevant for the outbreak dynamics, and to investigate the plasmids capability of horizontal
transfer, its segregational stability and plasmid-mediated fitness costs.
<p>Methods
Plasmid profiling was performed by S1-nuclease PFGE, PCR-based replicon typing and
Southern blot-hybridization. The complete sequence of the CTX-M-15-encoding plasmid
was obtained by 454 sequencing. Plasmid self-transferability was investigated by brothand
filter mating, segregational stability was explored by serial passage, and plasmidconferred
fitness costs were examined in pairwise head-to-head competitions and by
growth rate comparisons.
<p>Results
CTX-M-15 was encoded by a ~180 kb IncFIIK plasmid in K. pneumoniae ST17. S1-nuclease
PFGE profiles of the first and the last CTX-M-15-producing K. pneumoniae isolates, recovered
from the four children colonized the longest, suggested that the plasmid was stably
maintained during intestinal carriage of up to two years. The DNA sequence of the pKPN3-
like plasmid, pKp848CTX, uncovered a Tn3-like antibiotic resistance region and multiple
heavy metal- and thermoresistance determinants. Plasmid pKp848CTX could not be transferred to Escherichia coli in vitro and we found no evidence to support horizontal plasmid
transfer in vivo. Segregational plasmid loss ranging from 0.83% to 17.5% was demonstrated
in evolved populations in vitro, but only minor fitness costs were associated with
plasmid-carriage.
<p>Conclusions
Plasmid pKp848CTX encodes phenotypic traits, which may have had an impact on the fitness
and survival of the K. pneumoniae ST17 strain in the outbreak setting. The antibiotic
resistance plasmid pKp848CTX was stably maintained during two years of intestinal colonization,
conferring negligible fitness cost to its host, and thus seem well adapted to its K.
pneumoniae host. | en_US |
dc.identifier.citation | PLoS ONE 2015, 10(3): e0116516 | en_US |
dc.identifier.cristinID | FRIDAID 1254716 | |
dc.identifier.doi | 10.1371/journal.pone.0116516 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | https://hdl.handle.net/10037/8689 | |
dc.identifier.urn | URN:NBN:no-uit_munin_8178 | |
dc.language.iso | eng | en_US |
dc.publisher | Public Library of Science | en_US |
dc.relation.uri | http://www.plosone.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pone.0116516&representation=PDF | en_US |
dc.rights.accessRights | openAccess | |
dc.subject | VDP::Medisinske fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk mikrobiologi : 715 | en_US |
dc.subject | VDP::Midical sciences: 700::Basic medical, dental and veterinary sciences: 710::Medical microbiology: 715 | en_US |
dc.title | Persistence of a pKPN3-like CTX-M-15-encoding IncFIIK plasmid in a Klebsiella pneumonia ST17 host during two years of intestinal colonization | en_US |
dc.type | Journal article | en_US |
dc.type | Tidsskriftartikkel | en_US |
dc.type | Peer reviewed | en_US |