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dc.contributor.authorArya, Manit
dc.contributor.authorThrasivoulou, Christopher
dc.contributor.authorHenrique, Rui
dc.contributor.authorMillar, Michael
dc.contributor.authorHamblin, Ruth
dc.contributor.authorDavda, Reena
dc.contributor.authorAare, Kristina
dc.contributor.authorMasters, John R.
dc.contributor.authorThomson, Calum
dc.contributor.authorMuneer, Asif
dc.contributor.authorPatel, Hitendra R.H.
dc.contributor.authorAhmed, Aamir
dc.date.accessioned2016-08-31T08:23:34Z
dc.date.available2016-08-31T08:23:34Z
dc.date.issued2015-04-22
dc.description.abstractPenile squamous cell carcinoma (PeCa) is a rare malignancy and little is known regarding the molecular mechanisms involved in carcinogenesis of PeCa. The Wnt signaling pathway, with the transcription activator ß-catenin as a major transducer, is a key cellular pathway during development and in disease, particularly cancer. We have used PeCa tissue arrays and multi-fluorophore labelled, quantitative, immunohistochemistry to interrogate the expression of WNT4, a Wnt ligand, and three targets of Wnt-ß-catenin transcription activation, namely, MMP7, cyclinD1 (CD1) and c-MYC in 141 penile tissue cores from 101 unique samples. The expression of all Wnt signaling proteins tested was increased by 1.6 to 3 fold in PeCa samples compared to control tissue (normal or cancer adjacent) samples (p<0.01). Expression of all proteins, except CD1, showed a significant decrease in grade II compared to grade I tumors. High magnification, deconvolved confocal images were used to measure differences in co-localization between the four proteins. Significant (p<0.04-0.0001) differences were observed for various permutations of the combinations of proteins and state of the tissue (control, tumor grades I and II). Wnt signaling may play an important role in PeCa and proteins of the Wnt signaling network could be useful targets for diagnosis and prognostic stratification of disease.en_US
dc.description.sponsorshipThis research was funded by the Prostate Cancer Research Centre charity (registered UK charity no. 1156027). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en_US
dc.descriptionPublisher's version, source: <a href=http://dx.doi.org/10.1371/journal.pone.0124395>http://dx.doi.org/10.1371/journal.pone.0124395 </a>.en_US
dc.identifier.citationPLoS ONE 2015, 10(4):e0124395en_US
dc.identifier.cristinIDFRIDAID 1345974
dc.identifier.doi10.1371/journal.pone.0124395
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/10037/9601
dc.identifier.urnURN:NBN:no-uit_munin_9149
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.rights.accessRightsopenAccess
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Onkologi: 762en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Cellebiologi: 471en_US
dc.titleTargets of Wnt/ß-Catenin Transcription in Penile Carcinomaen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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