English
norskBlar i forfatter "Basnet, Purusotam"
Viser treff 21-32 av 32
-
Localized Therapy of Vaginal Infections and Inflammation: Liposomes-In-Hydrogel Delivery System for Polyphenols
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-01-27)Natural polyphenols, such as resveratrol (RES) or epicatechin (EPI), are attractive for treatments of various diseases, including vaginal infections and inflammation, because of their strong anti-oxidative and anti-inflammatory properties. However, their low solubility and consequent poor bioavailability limit their therapeutic uses. To overcome these limitations, a vaginal delivery system comprising ... -
Multi-modal chip-based fluorescence and quantitative phase microscopy for studying inflammation in macrophages
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-07-24)Total internal reflection fluorescence (TIRF) microscopy benefits from high-sensitivity, low background noise, low photo-toxicity and high-contrast imaging of sub-cellular structures close to the membrane surface. Although, TIRF microscopy provides high-contrast imaging it does not provide quantitative information about morphological features of the biological cells. Here, we propose an integrated ... -
Nitric oxide, oxidative stress and streptococcus mutans and lactobacillus bacterial loads in saliva during the different stages of pregnancy: A longitudinal study
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-09-03)Hormonal changes associated with pregnancy promote oral bacterial growth, which may affect salivary nitric oxide (NO) levels, oxidative stress (OS), and antioxidant capacity (AC). We hypothesized that caries-related bacterial load, NO level, and OS in the saliva change with advancing gestation. The aim of this study was to investigate longitudinal changes in salivary NO, OS, and AC during pregnancy ... -
Oxidative stress levels and oral bacterial milieu in the saliva from pregnant vs. non-pregnant women
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-09-03)<i>Background</i> - Saliva plays a significant role in maintaining oral health and oral bacterial milieu. Difference in oxidative stress (OS) levels in saliva in conjunction with bacterial load between pregnant and non-pregnant women has not been studied previously. We hypothesized that the physiological changes in pregnancy alter oral bacterial milieu by promoting growth of <i>Streptococcus mutans</i> ... -
Partially spatially coherent digital holographic microscopy and machine learning for quantitative analysis of human spermatozoa under oxidative stress condition
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-03-05)Semen quality assessed by sperm count and sperm cell characteristics such as morphology and motility, is considered to be the main determinant of men’s reproductive health. Therefore, sperm cell selection is vital in assisted reproductive technology (ART) used for the treatment of infertility. Conventional bright field optical microscopy is widely utilized for the imaging and selection of sperm cells ... -
PEGylated liposomes for topical vaginal therapy improve delivery of interferon alpha
(Journal article; Tidsskriftartikkel; Peer reviewed, 2017-01-11)Recent studies regarding mucosal drug delivery indicate that nanosystems with surface-available polyethylene glycol (PEG) are able to penetrate mucus barrier, assure closer contact with the epithelium, and improve drug delivery to vagina. In the present work, we developed the mucus-penetrating PEGylated liposomes containing interferon alpha-2b (IFN α-2b), destined to provide localized therapy for ... -
Quantitative assessment of morphology and sub-cellular changes in macrophages and trophoblasts during inflammation
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-06-12)In pregnancy during an inflammatory condition, macrophages present at the feto-maternal junction release an increased amount of nitric oxide (NO) and pro-inflammatory cytokines such as TNF-α and INF-γ, which can disturb the trophoblast functions and pregnancy outcome. Measurement of the cellular and sub-cellular morphological modifications associated with inflammatory responses are important in order ... -
Resveratrol-loaded liposomes for topical treatment of the vaginal inflammation and infections
(Journal article; Tidsskriftartikkel; Peer reviewed, 2015-09-07)Resveratrol (RES), chemically known as 3,5,4′-trihydroxy-trans-stilbene, is a promising multi-targeted antioxidative and anti-inflammatory natural polyphenol. Preclinical studies showed its biological activities against the pathogens of sexually transmitted diseases causing vaginal inflammation and infections. Due to its low solubility and poor bioavailability, the optimal therapeutic uses are ... -
Sperm mitochondrial DNA copy numbers in normal and abnormal semen analysis: A systematic review and meta-analysis
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-12-25)Background: Normal mature sperm have a considerably reduced number of mitochondria, which provide the energy required for progressive sperm motility. Literature suggests that disorders of sperm motility may be linked to abnormal sperm mitochondrial number and function.<p> <p>Objectives: To summarise the evidence from literature regarding the association of mitochondrial DNA copy numbers and semen ... -
Tailored anti-biofilm activity – Liposomal delivery for mimic of small antimicrobial peptide
(Journal article; Tidsskriftartikkel; Peer reviewed, 2023-12-13)The eradication of bacteria embedded in biofilms is among the most challenging obstacles in the management of chronic wounds. These biofilms are found in most chronic wounds; moreover, the biofilm-embedded bacteria are considerably less susceptible to conventional antimicrobial treatment than the planktonic bacteria. Antimicrobial peptides and their mimics are considered attractive candidates in the ... -
Utilizing Liposomal Quercetin and Gallic Acid in Localized Treatment of Vaginal Candida Infections
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-12-20)Vulvovaginal candidiasis (VVC) is a widely spread fungal infection that causes itching, pain and inflammation at the vaginal site. Although common, currently available treatment suffers from limited efficacy and high recurrence. In addition, the growing problem of resistance to azole drugs used in current treatments emphasizes the need for superior treatment options. Antimicrobial polyphenols are ... -
Visualizing ultrastructural details of placental tissue with super-resolution structured illumination microscopy
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-06-14)Super-resolution fluorescence microscopy is a widely employed technique in cell biology research, yet remains relatively unexplored in the field of histopathology. Here, we describe the sample preparation steps and acquisition parameters necessary to obtain fluorescent multicolor super-resolution structured illumination microscopy (SIM) images of both formalin-fixed paraffin-embedded and cryo-preserved ...
