Blar i forfatter "Smedsrød, Bård"

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    • Protocol for Isolation and Culture of Mouse Hepatocytes (HCs), Kupffer Cells (KCs), and Liver Sinusoidal Endothelial Cells (LSECs) in Analyses of Hepatic Drug Distribution 

      Elvevold, Kjetil; Kyrrestad, Ingelin; Smedsrød, Bård (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-02-25)
      Development of the new generation of drugs (e.g., oligo- and polynucleotides administered intravascularly either as free compounds or as nano-formulations) frequently encounters major challenges such as lack of control of targeting and/or delivery. Uncontrolled or unwanted clearance by the liver is a well-known and particularly important hurdle in this respect. Hence, reliable techniques are needed ...

    • Protocol for preparation of mouse liver Kupffer cells and liver sinusoidal endothelial cells 

      Smedsrød, Bård (Others; Andre, 2012)
      The following protocol is an outline of the method we use to prepare Kupffer cells (KC) and liver sinusoidal endothelial cells (LSEC) from mouse liver. The method is based on the one originally published to prepare rat liver KC and LSEC (1), and is slightly modified from the one outlined in (2). We also strongly recommend reading our 2012 review paper (3) to obtain a solid background understanding ...

    • Pulmonary vascular clearance of harmful endogenous macromolecules in a porcine model of acute liver failure 

      Nedredal, Geir Ivar; Elvevold, Kjetil Helge; Chedid, MF; Ytrebø, Lars Marius; Rose, CF; Sen, S; Smedsrød, Bård; Jalan, R; Revhaug, Arthur (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-05-30)
      Background. Pulmonary complications are common in acute liver failure (ALF). The role of the lungs in the uptake of harmful soluble endogenous macromolecules was evaluated in a porcine model of ALF induced by hepatic devascularization (n = 8) vs. controls (n = 8). In additional experiments, pulmonary uptake was investigated in healthy pigs. Fluorochrome-labeled modified albumin (MA) was applied to ...

    • Rat liver sinusoidal endothelial cells (LSECs) express functional low density lipoprotein receptor-related protein-1 (LRP-1) 

      Øie, Cristina Ionica; Appa, Rupa Shree; Hilden, Ida; Petersen, Helle Heibroch; Gruhler, Albrecht; Smedsrød, Bård; Hansen, John-Bjarne (Journal article; Tidsskriftartikkel; Peer reviewed, 2011)
      The low density lipoprotein receptor-related protein-1 (LRP-1) is a large, multifunctional endocytic receptor from the LDL receptor family, highly expressed in liver parenchymal cells (PCs), neurons, activated astrocytes, and fibroblasts. The aim of the study was to investigate if liver sinusoidal endothelial cells (LSECs), highly specialized scavenger cells, express LRP-1. To address this question, ...

    • The Relationship between Fenestrations, Sieve Plates and Rafts in Liver Sinusoidal Endothelial Cells 

      Svistounov, Dmitri; Warren, Alessandra; McNerney, Gregory P; Owen, Dylan M.; Zencak, Dusan; Zykova, Svetlana N; Crane, Harry; Huser, Thomas; Quinn, Ronald J.; Smedsrød, Bård; Le Couteur, David G; Cogger, Victoria C (Journal article; Tidsskriftartikkel; Peer reviewed, 2012)
      Fenestrations are transcellular pores in endothelial cells that facilitate transfer of substrates between blood and the extravascular compartment. In order to understand the regulation and formation of fenestrations, the relationship between membrane rafts and fenestrations was investigated in liver sinusoidal endothelial cells where fenestrations are grouped into sieve plates. Three dimensional ...

    • The scavenger function of liver sinusoidal endothelial cells in health and disease 

      Bhandari, Sabin; Larsen, Anett Kristin; McCourt, Peter Anthony; Smedsrød, Bård; Sørensen, Karen Kristine (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-10-11)
      The aim of this review is to give an outline of the blood clearance function of the liver sinusoidal endothelial cells (LSECs) in health and disease. Lining the hundreds of millions of hepatic sinusoids in the human liver the LSECs are perfectly located to survey the constituents of the blood. These cells are equipped with high-affinity receptors and an intracellular vesicle transport apparatus, ...

    • Scavenger properties of cultivated pig liver endothelial cells 

      Elvevold, Kjetil H.; Nedredal, Geir I.; Revhaug, Arthur; Smedsrød, Bård (Journal article; Tidsskriftartikkel; Peer reviewed, 2004-08-12)
      Background: The liver sinusoidal endothelial cells (LSEC) and Kupffer cells constitute the most powerful scavenger system in the body. Various waste macromolecules, continuously released from tissues in large quantities as a consequence of normal catabolic processes are cleared by the LSEC. In spite of the fact that pig livers are used in a wide range of experimental settings, the scavenger ...

    • Stans gratisarbeidet for grådige tidsskriftsforlag 

      Longva, Leif; Smedsrød, Bård (Journal article; Tidsskriftartikkel, 2021-12-26)
      I en tid med stramme budsjetter bør både Riksrevisjonen og alle ledere i universitets- og høgskolesektoren interessere seg for sløseriet av midler som går til overprisede tidsskrifter.

    • Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages 

      Bhandari, Sabin; Li, Ruomei; Simon-Santamaria, Jaione; McCourt, Peter Anthony; Johansen, Steinar Daae; Smedsrød, Bård; Martinez, Inigo Zubiavrre; Sørensen, Karen Kristine (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-11-27)
      <i>Background</i> - Liver sinusoidal endothelial cells (LSECs) and Kupffer cells (KCs; liver resident macrophages) form the body’s most effective scavenger cell system for the removal of harmful blood-borne substances, ranging from modified self-proteins to pathogens and xenobiotics. Controversies in the literature regarding the LSEC phenotype pose a challenge when determining distinct functionalities ...

    • Uptake and Degradation of Bacteriophages by Liver Sinusoidal Endothelial Cells 

      Wolfson, Deanna; Øie, Cristina Ionica; Yasunori, Tanji; Dumitriu, Gianina; McCourt, Peter Anthony; Sørensen, Karen Kristine; Smedsrød, Bård; Ahluwalia, Balpreet Singh (Conference object; Konferansebidrag, 2018)
      <p>Bacteriophages (briefly, “phages”) are viruses which target bacteria, and are non-infectious to eukaryotic cells. It is estimated that more than 30 billion phages cross into the human body from the gut each day1, and eventually need to be cleared from the blood circulation. The liver plays a central role in pathogen clearance, and liver sinusoidal endothelial cells (LSECs), which form the lining ...

    • Vitenskapelige tidsskrifter: makten og æren 

      Longva, Leif; Smedsrød, Bård (Journal article; Tidsskriftartikkel, 2010)

    • Voldsom sløsing med offentlige penger i vitenskapelig publisering 

      Smedsrød, Bård; Longva, Leif; Frantsvåg, Jan Erik (Chronicle; Kronikk, 2018-02-27)
      <i>Ingress</i>: Offentlig finansierte forskere skriver og kvalitetssikrer vitenskapelige artikler. Så tar et tidsskrift over alle økonomiske rettigheter. I neste omgang må forskerne kjøpe tilbake adgangen til de samme artiklene.