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norskBlar i forfatter "Moens, Ugo"
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Regulation of Transcriptional Activity of Merkel Cell Polyomavirus Large T-Antigen by PKA-Mediated Phosphorylation
(Journal article; Tidsskriftartikkel; Peer reviewed, 2023-01-03)Merkel cell polyomavirus (MCPyV) is the major cause of Merkel cell carcinoma (MCC), an aggressive skin cancer. MCPyV large T-antigen (LTag) and small T-antigen (sTag) are the main oncoproteins involved in MCPyV-induced MCC. A hallmark of MCPyV-positive MCC cells is the expression of a C-terminal truncated LTag. Protein kinase A (PKA) plays a fundamental role in a variety of biological processes, ... -
Reply to Henriksen, S.; Rinaldo, C.H. Should SVGp12 Be Used for JC Polyomavirus Studies? Comment on “Prezioso et al. COS-7 and SVGp12 Cellular Models to Study JCPyV Replication and MicroRNA Expression after Infection with Archetypal and Rearranged-NCCR Viral Strains. Viruses 2022, 14, 2070”
(Journal article; Tidsskriftartikkel; Peer reviewed, 2022-12-29)In relation to the comment by Henriksen and Rinaldo, the authors intend to emphasize that before every experiment with SVGp12 cells they routinely test the cells for the absence of BKPyV contamination. The scientists can state that the SVGp12 cells used in their laboratory were not infected by BKPyV and that their results were also validated on the COS-7 cell line, which is permissive for JCPyV ... -
The role of Merkel cell polyomavirus and other human polyomaviruses in emerging hallmarks of cancer
(Journal article; Tidsskriftartikkel; Peer reviewed, 2015-04-10)Polyomaviruses are non-enveloped, dsDNA viruses that are common in mammals, including humans. All polyomaviruses encode the large T-antigen and small t-antigen proteins that share conserved functional domains, comprising binding motifs for the tumor suppressors pRb and p53, and for protein phosphatase 2A, respectively. At present, 13 different human polyomaviruses are known, and for some of them ... -
A role of Sp1 binding motifs in basal and large T-Antigen-induced promoter activities of human polyomavirus HPyV9 and its variant UF-1
(Journal article; Tidsskriftartikkel; Peer reviewed, 2017-11-14)Human polyomavirus 9 (HPyV9) was originally detected in the serum of a renal transplant patient. Seroepidemiological studies showed that ~20-50% of the human population have antibodies against this virus. HPyV9 has not yet been associated with any disease and little is known about the route of infection, transmission, host cell tropism, and genomic variability in circulating strains. Recently, the ... -
Role of Virus-Induced Host Cell Epigenetic Changes in Cancer
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-08-03)The tumor viruses human T-lymphotropic virus 1 (HTLV-1), hepatitis C virus (HCV), Merkel cell polyomavirus (MCPyV), high-risk human papillomaviruses (HR-HPVs), Epstein-Barr virus (EBV), Kaposi’s sarcoma-associated herpes virus (KSHV) and hepatitis B virus (HBV) account for approximately 15% of all human cancers. Although the oncoproteins of these tumor viruses display no sequence similarity to one ... -
Septin 8 is an interaction partner and in vitro substrate of MK5.
(Journal article; Tidsskriftartikkel; Peer reviewed, 2012)To identify novel substrates for the mitogen-activated protein kinase-activated protein kinase 5 (MK5). Yeast two-hybrid screening with MK5 as bait was used to identify novel possible interaction partners. The binding of putative partner was further examined by glutathione S-transferase (GST) pull-down, co-immunoprecipitation and fluorescence resonance energy transfer (FRET) analysis. In vitro kinase ... -
Serine residue 115 of MAPK-activated protein kinase MK5 is crucial for its PKA-regulated nuclear export and biological function.
(Journal article; Tidsskriftartikkel; Peer reviewed, 2010) -
Silencing Viral MicroRNA as a Novel Antiviral Therapy?
(Journal article; Tidsskriftartikkel; Peer reviewed, 2009-03-20)Viruses are intracellular parasites that ensure their existence by converting host cells into viral particle producing entities or into hiding places rendering the virus invisible to the host immune system. Some viruses may also survive by transforming the infected cell into an immortal tumour cell. MicroRNAs are small non-coding transcripts that function as posttranscriptional regulators of gene ... -
Structural Analysis of Merkel Cell Polyomavirus (MCPyV) Viral Capsid Protein (VP1) in HIV-1 Infected Individuals
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-10-27)Merkel cell polyomavirus (MCPyV) viral protein 1 (VP1) is the capsid protein that mediates virus attachment to host cell receptors and is the major immune target. Given the limited data on MCPyV VP1 mutations, the VP1 genetic variability was examined in 100 plasma and 100 urine samples from 100 HIV+ individuals. Sequencing of VP1 DNA in 17 urine and 17 plasma specimens, simultaneously MCPyV DNA ... -
SYK Inhibition Potentiates the Effect of Chemotherapeutic Drugs on Neuroblastoma Cells in Vitro
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-02-10)Neuroblastoma is a malignancy arising from the developing sympathetic nervous system and the most common and deadly cancer of infancy. New therapies are needed to improve the prognosis for high-risk patients and to reduce toxicity and late effects. Spleen tyrosine kinase (SYK) has previously been identified as a promising drug target in various inflammatory diseases and cancers but has so far not ... -
A taxonomy update for the Family Polyomaviridae
(Journal article; Tidsskriftartikkel; Peer reviewed, 2016-02-29)Many distinct polyomaviruses infecting a variety of vertebrate hosts have recently been discovered, and their complete genome sequence could often be determined. To accommodate this fast-growing diversity, the International Committee on Taxonomy of Viruses (ICTV) <i>Polyomaviridae</i> Study Group designed a host- and sequence-based rationale for an updated taxonomy of the family <i>Polyomaviridae</i>. ... -
Transgenic mice expressing constitutive active MAPKAPK5 display gender-dependent differences in exploration and activity
(Journal article; Tidsskriftartikkel; Peer reviewed, 2007-11-12)Background The mitogen-activated protein kinases, MAPKs for short, constitute cascades of signalling pathways involved in the regulation of several cellular processes that include cell proliferation, differentiation and motility. They also intervene in neurological processes like fear conditioning and memory. Since little remains known about the MAPK-Activated Protein Kinase, MAPKAPK5, we constructed ... -
Tumour promoting and suppressing roles of the atypical MAP kinase signalling pathway ERK3/4-MK5.
(Journal article; Tidsskriftartikkel; Peer reviewed, 2012)Perturbed action of signal transduction pathways, including the mitogen-activated protein (MAP) kinase pathways, is one of the hallmarks of many cancers. While the implication of the typical MAP kinase pathways ERK1/2-MEK1/2, p38MAPK and JNK is well established, recent findings illustrate that the atypical MAP kinase ERK3/4-MK5 may also be involved in tumorigenic processes. Remarkably, the ERK3/4-MK5 ...
