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dc.contributor.authorFlorholmen-Kjær, Åse
dc.contributor.authorGoll, Rasmus
dc.contributor.authorFuskevåg, Ole-Martin
dc.contributor.authorNygård, Ingvild
dc.contributor.authorPaulssen, Ruth H
dc.contributor.authorRevhaug, Arthur
dc.contributor.authorMortensen, Kim Erlend
dc.date.accessioned2017-02-16T09:46:53Z
dc.date.available2017-02-16T09:46:53Z
dc.date.issued2016-05-24
dc.description.abstractBackground Previous studies on oxidative state after partial hepatectomy (PHx) report conflicting data on levels of glutathione (GSH) and are mainly presented in rodent models by methodology less sensitive than the present technologies. The current swine model presents GSH levels and the following genetic response post-PHx, utilizing an analytical platform more sensitive and precise than earlier available. Method Twelve pigs were randomized to a PHx- and a control group (n=6 in each). The PHx group had a 60% hepatectomy. Serial in vivo liver biopsies during 12 h of anaesthesia post-PHx were analyzed for GSH by liquid chromatography mass spectrometry (LC-MS/MS). Transcriptional alterations of genes (GS, GCLM, GCLC, GR, HGF, NFE2L2, TGFβ1) regulating GSH synthesis were measured by real-time PCR. Results No difference was detected between the GSH levels in the PHx- and the control group during the experiment (P=0.247). Still, decreased gene expression of GS (P=0.026) and NFE2L2 (P=0.014) the first nine hours, and a decrease of TGFβ1 (P=0.029) the first seven hours post-PHx was seen in the liver remnant. Conclusion The results show that the liver has an extended capacity to maintain GSH homeostasis during major stress and parenchymal loss, even at the early onset of such trauma. This observation was not explained by increased expression of key genes in GSH pathways. Consequently, the results indicate an inherent compensatory capacity to maintain GSH homeostasis in the reduced organ.en_US
dc.identifier.citationÅse Florholmen-Kjær, Rasmus Goll, Ole-Martin Fuskevåg, Ingvild Engdal Nygård, Ruth H. Paulssen, Arthur Revhaug, Kim Erlend Mortensen, The impact of partial hepatectomy on oxidative state in the liver remnant – An in vivo swine model, Redox Biology, Volume 9, October 2016, Pages 15-21, ISSN 2213-2317, http://dx.doi.org/10.1016/j.redox.2016.05.005.en_US
dc.identifier.cristinIDFRIDAID 1371271
dc.identifier.doi10.1016/j.redox.2016.05.005
dc.identifier.issn2213-2317
dc.identifier.urihttps://hdl.handle.net/10037/10301
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalRedox Biology
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medisinske Fag: 700en_US
dc.subjectVDP::Medical disciplines: 700en_US
dc.subjectGlutathioneen_US
dc.subjectPartial hepatectomyen_US
dc.subjectLiquid chromatography mass spectrometry/LC-MS/MSen_US
dc.subjectSwineen_US
dc.subjectOxidative stateen_US
dc.titleThe impact of partial hepatectomy on oxidative state in the liver remnant - An in vivo swine modelen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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