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dc.contributor.authorIslam, Md Ashraful
dc.contributor.authorHansen, Ann Kristin
dc.contributor.authorMennan, Claire
dc.contributor.authorMartinez, Inigo Zubiavrre
dc.date.accessioned2017-03-10T11:49:51Z
dc.date.available2017-03-10T11:49:51Z
dc.date.issued2016-05-27
dc.description.abstractMany researchers world over are currently investigating the suitability of stromal cells harvested from foetal tissues for allogeneic cell transplantation therapies or for tissue engineering purposes. In this study, we have investigated the chondrogenic potential of mesenchymal stromal cells (MSCs) isolated from whole sections of human umbilical cord or mixed cord (UCSCs-MC), and compared them with cells isolated from synovial membrane (SMSCs), Hoffa’s fat pad (HFPSCs) and cartilage. All MSCs were positive for surface markers including CD73, CD90, CD105, CD44, CD146 and CD166, but negative for CD11b, CD19, CD34, CD45 and HLA-DR in addition to CD106 and CD271. Chondrogenic potential of all cell sources was studied using 3D pellet cultures incubated in the presence of different combinations of anabolic substances such as dexamethasone, IGF-1, TGF-β1, TGF-β3, BMP-2 and BMP-7. BMP-2 and dexamethasone in combination with TGF-β1 or TGF-β3 excelled at inducing chondrogenesis on SMSCs, HFPSCs and chondrocytes, as measured by glycosaminoglycans and collagen type II staining of pellets, quantitative glycosaminoglycan expression, quantitative PCR of cartilage signature genes and electron microscopy. In contrast, none of the tested growth factor combinations was sufficient to induce chondrogenesis on UCSCs-MC. Moreover, incubation of UCSCs-MC spheroids in the presence of cartilage pieces or synovial cells in co-cultures did not aid chondrogenic induction. In summary, we show that in comparison with MSCs harvested from adult joint tissues, UCSCs-MC display poor chondrogenic abilities. This observation should alert researchers at the time of considering UCSCs-MC as cartilage forming cells in tissue engineering or repair strategies.en_US
dc.description.sponsorshipThis work is supported by University of Tromsø and Arthritis Research UK (CM, via grant No. 19429).en_US
dc.descriptionSource at <a href=http://dx.doi.org/10.22203/eCM.v031a26>http://dx.doi.org/10.22203/eCM.v031a26</a>en_US
dc.identifier.citationIslam, A., Hansen, A.K., Mennan, C. & Martinez-Zubiaurre, I. (2016). Mesenchymal stromal cells from human umbilical cords display poor chondrogenic potential in scaffold-free three dimensional cultures. <i>European Cells and Materials, 31</i>, 407-424. http://dx.doi.org/10.22203/eCM.v031a26en_US
dc.identifier.cristinIDFRIDAID 1371108
dc.identifier.doiDOI: 10.22203/eCM.v031a26
dc.identifier.issn1473-2262
dc.identifier.urihttps://hdl.handle.net/10037/10544
dc.language.isoengen_US
dc.publisherAO Research Institute Davosen_US
dc.relation.ispartofIslam, A. (2018). Biology of mesenchymal stromal cells: Chondrogenesis, paracrine signalling and cartilage repair. Doctoral thesis. <a href=http://hdl.handle.net/10037/14118>http://hdl.handle.net/10037/14118 </a>
dc.relation.journalEuropean Cells and Materials
dc.relation.urihttp://www.ecmjournal.org/journal/papers/vol031/pdf/v031a26.pdf
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750en_US
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750en_US
dc.titleMesenchymal stromal cells from human umbilical cords display poor chondrogenic potential in scaffold-free three dimensional culturesen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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