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dc.contributor.authorNystad, Mona
dc.contributor.authorSitras, Vasilis
dc.contributor.authorFlo, Kari
dc.contributor.authorWidnes, Christian
dc.contributor.authorVårtun, Åse
dc.contributor.authorWilsgaard, Tom
dc.contributor.authorAcharya, Ganesh
dc.date.accessioned2017-03-14T14:52:53Z
dc.date.available2017-03-14T14:52:53Z
dc.date.issued2016-11-25
dc.description.abstractBackground: Laeverin is a placenta-specific membrane-bound aminopeptidase. In this study we wanted to: 1) serially measure plasma levels of laeverin in healthy women during the second half of pregnancy and postpartum, 2) determine whether laeverin is differently expressed at 22–24 weeks in women who later develop preeclampsia compared to controls, 3) compare laeverin protein expression in placenta and umbilical vein serum in healthy and preeclamptic pregnancies at birth. Methods: Plasma was obtained serially, approximately every 4-weeks, from 53 healthy women with uncomplicated pregnancies during 22+0 to 39+6 weeks of gestation, and at 22–24 weeks from 15 women who later developed preeclampsia. Enzyme-linked immunosorbent assay was used to measure laeverin protein concentration. Serum from healthy non-pregnant premenopausal women (n = 10), menopausal women (n = 10) and men (n = 11) were used as negative controls. Protein extracts from placental tissue were obtained after birth from healthy- (n = 11) and preeclamptic women (n = 13). Paired umbilical artery and vein serum samples from the neonates (n = 10) of healthy mothers were also analyzed. Multilevel modeling was used to determine the reference centiles. Differences between groups were analyzed using Student’s t-test. Results: Healthy pregnant women at term (37–40 weeks) had significantly higher plasma levels of laeverin (mean 4.95 ± 0.32 ng/mL; p < 0.0001) compared to men (mean 0.18 ± 0.31 ng/mL), non-pregnant premenopausal women (mean 0.77 ± 0.26 ng/mL) and postmenopausal women (mean 0.57 ± 0.40 ng/mL). Maternal plasma laeverin levels decreased with advancing gestation, from 6.96 ± 0.32 ng/mL at 22–24 weeks to 4.95 ± 0.32 ng/mL at term (p < 0. 0001) in uncomplicated pregnancies. Half of the women who developed preeclampsia had plasma laeverin levels below the 5th percentile at 22–24 weeks gestation. However, laeverin levels were 1.6 fold higher in preeclamptic compared to healthy placentas (p = 0.0071). Umbilical venous samples of healthy neonates (n = 38) had higher (p = 0.001) mean levels of laeverin (16.63 ± 0.73 ng/mL), compared to neonates of preeclamptic (n = 14) mothers (12.02 ± 1.00 ng/mL). Postpartum plasma levels of laeverin decreased in healthy and preeclamptic women with a half-life of 3 and 5 days, respectively. Conclusions: Maternal plasma levels of laeverin decrease with advancing gestation during the second half of normal pregnancy and lower levels measured at 22–24 weeks might be associated with the development of preeclampsia later in gestation.en_US
dc.descriptionPublished version: http://dx.doi.org/10.1186/s12884-016-1156-9en_US
dc.identifier.citationNystad M, Sitras VS, Flo K, Widnes C, Vårtun Å, Wilsgaard T, Acharya G. Longitudinal reference ranges for maternal plasma laeverin, and its role as a potential biomarker of preeclampsia. BMC Pregnancy and Childbirth. 2016;16(1)en_US
dc.identifier.cristinIDFRIDAID 1404652
dc.identifier.doi10.1186/s12884-016-1156-9
dc.identifier.issn1471-2393
dc.identifier.urihttps://hdl.handle.net/10037/10668
dc.language.isoengen_US
dc.publisherBioMed Centralen_US
dc.relation.journalBMC Pregnancy and Childbirth
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Gynekologi og obstetrikk: 756en_US
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750::Gynecology and obstetrics: 756en_US
dc.subjectAminopeptidaseen_US
dc.subjectAminopeptidase-Qen_US
dc.subjectELISAen_US
dc.subjectLaeverinen_US
dc.subjectPlasmaen_US
dc.subjectPreeclampsiaen_US
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750en_US
dc.titleLongitudinal reference ranges for maternal plasma laeverin, and its role as a potential biomarker of preeclampsiaen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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