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dc.contributor.authorIngebrigtsen, Sveinung Gaarden
dc.contributor.authorSkalko-Basnet, Natasa
dc.contributor.authorCavalcanti Jacobsen, Cristiane de Albuquerque
dc.contributor.authorHolsæter, Ann Mari
dc.date.accessioned2017-03-27T10:13:43Z
dc.date.available2017-03-27T10:13:43Z
dc.date.issued2017-11-16
dc.description.abstractEncapsulation of more than one active pharmaceutical ingredient into nanocarriers such as liposomes is an attractive approach to achieve a synergic drug effect and less complicated dosing schedules in multi-drug treatment regimes. Liposomal drug delivery in acne treatment may improve drug efficiency by targeted delivery to pilosebaceous units, reduce adverse effects and improve patient compliance. We therefore aimed to co-encapsulate benzoyl peroxide (BPO) and chloramphenicol (CAM) into liposomes using the novel liposome processing method – dual asymmetric centrifugation (DAC). Liposomes were formed from soybean lecithin, propylene glycol and distilled water (2:1:2 w/v/v ratio), forming a viscous liposome dispersion. Liposomes containing both drugs (BPO-CAM-Lip), single-drug (BPO-Lip and CAM-Lip), and empty liposomes were prepared. Drug entrapment of BPO and CAM was determined by a newly developed HPLC method for simultaneous detection and quantification of both drugs. Encapsulation of around 50% for BPO and 60% for CAM respectively was obtained in both single- drug encapsulated formulations (BPO-Lip and CAM-Lip) and co-encapsulated formulations (BPO- CAM-Lip). Liposome sizes were comparable for all liposome formulations, ranging from 130 to 150 nm mean diameter, with a polydispersity index < 0.2 for all formulations. CAM exhibited a sustained release from all liposomal formulations, whereas BPO appeared retained within the liposomes. BPO retention could be attributed to its poor solubility. However, HaCaT cell toxicity was found dependent on BPO released from the liposomes. In the higher concentration range (4% v/v), liposomal formulations were less cytotoxic than the corresponding drug solutions used as reference. We have demonstrated that DAC is a fast, easy, suitable method for encapsulation of more than one drug within the same liposomes.en_US
dc.descriptionAccepted manuscript version. Published version available at <a href=http://dx.doi.org/10.1016/j.ejps.2016.11.017> http://dx.doi.org/10.1016/j.ejps.2016.11.017 </a>en_US
dc.identifier.citationIngebrigtsen SG, Skalko-Basnet N, Cavalcanti Jacobsen CDAC, Holsæter A M. Successful co-encapsulation of benzoyl peroxide and chloramphenicol in liposomes by a novel manufacturing method - dual asymmetric centrifugation. European Journal of Pharmaceutical Sciences. 2017;97:192-199en_US
dc.identifier.cristinIDFRIDAID 1401422
dc.identifier.doi10.1016/j.ejps.2016.11.017
dc.identifier.issn0928-0987
dc.identifier.issn1879-0720
dc.identifier.urihttps://hdl.handle.net/10037/10872
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalEuropean Journal of Pharmaceutical Sciences
dc.relation.urihttp://www.sciencedirect.com/science/article/pii/S0928098716305103
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Farmakologi: 728en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Pharmacology: 728en_US
dc.subjectDual asymmetric centrifugationen_US
dc.subjectCo-encapsulationen_US
dc.subjectLiposomesen_US
dc.subjectBenzoyl peroxideen_US
dc.subjectAcneen_US
dc.titleSuccessful co-encapsulation of benzoyl peroxide and chloramphenicol in liposomes by a novel manufacturing method - dual asymmetric centrifugationen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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