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dc.contributor.authorLi, Chun
dc.contributor.authorBlencke, Hans-Matti
dc.contributor.authorHaug, Tor
dc.contributor.authorJørgensen, Øyvind
dc.contributor.authorStensvåg, Klara
dc.date.accessioned2017-12-09T10:36:28Z
dc.date.available2017-12-09T10:36:28Z
dc.date.issued2013-11-12
dc.description.abstractAntimicrobial peptides (AMPs) play a crucial role in innate immunity. We have previously reported the isolation and characterization of the AMPs, strongylocins 1 and 2, and centrocin 1, from coelomocyte extracts of Strongylocentrotus droebachiensis. Here we show that these AMPs were expressed in phagocytes. In addition, transcripts of strongylocin 1 were detected in vibratile cells and/or colorless spherule cells, while transcripts of strongylocin 2 were found in red spherule cells. Results from immunoblotting and immunocytochemistry studies showed that centrocin 1 was produced by phagocytes and stored in granular vesicles. Co-localization of centrocin 1 and phagocytosed bacteria suggests that the granular vesicles containing centrocin 1 may be involved in the formation of phagolysosomes. We also analyzed the temporal and spatial expression of AMPs throughout larval development. Strongylocins were expressed in the early pluteus stage, while centrocin 1 was expressed in the mid pluteus stage. The spatial expression pattern showed that centrocin 1 was mainly located in blastocoelar cells (BCs) around the stomach and the esophagus. In addition, a few patrolling BCs were detected in some larval arms. Together, these results suggest that AMPs are expressed in different types of coelomocytes and that centrocin 1 is involved in response against bacteria. Furthermore, the expression of AMPs in larval pluteus stage, especially in BCs, indicates that AMPs and BCs are engaged in the larval immune system.en_US
dc.identifier.citationLi C, Blencke H, Haug T, Jørgensen, Stensvåg K. Expression of antimicrobial peptides in coelomocytes and embryos of the green sea urchin (Strongylocentrotus droebachiensis). Developmental and Comparative Immunology. 2014;43(1):106-113en_US
dc.identifier.cristinIDFRIDAID 1061353
dc.identifier.doi10.1016/j.dci.2013.10.013
dc.identifier.issn0145-305X
dc.identifier.issn1879-0089
dc.identifier.urihttps://hdl.handle.net/10037/11832
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.ispartofLi, C. (2022). Antimicrobial peptides in sea urchins. Isolation, characterization and expression. (Doctoral thesis). <a href=https://hdl.handle.net/10037/25507>https://hdl.handle.net/10037/25507</a>
dc.relation.journalDevelopmental and Comparative Immunology
dc.relation.projectIDAndre: Tromsø Forskningsstiftelseen_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/HAVKYST/178214/Norway/Antibiotics from the sea - isolation and characterization of novel compounds from cold-water benthic organisms//en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/HAVKYST/184688/Norway/ Antibiotics from the sea - a MabCent project//en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/SFI/174885/Norway/MabCent - Centre on Marine Bioactives and Drug Discovery//en_US
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk immunologi: 716en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical immunology: 716en_US
dc.titleExpression of antimicrobial peptides in coelomocytes and embryos of the green sea urchin (Strongylocentrotus droebachiensis)en_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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