Show simple item record

dc.contributor.authorOral, Münevver
dc.contributor.authorColléter, Julie
dc.contributor.authorBekaert, Michaël
dc.contributor.authorTaggart, John B.
dc.contributor.authorPalaoikostas, Christos
dc.contributor.authorMcAndrew, Brendan J.
dc.contributor.authorVandeputte, Marc
dc.contributor.authorChatain, Béatrice
dc.contributor.authorKuhl, Heiner
dc.contributor.authorReinhardt, Richard
dc.contributor.authorPeruzzi, Stefano
dc.contributor.authorPenman, David J.
dc.date.accessioned2018-02-14T10:37:32Z
dc.date.available2018-02-14T10:37:32Z
dc.date.issued2017-06-07
dc.description.abstractBackground: <br> Fully isogenic lines in fish can be developed using “mitotic” gynogenesis (suppression of first zygotic mitosis following inactivation of the sperm genome). However, genome-wide verification of the steps in this process has seldom been applied. We used ddRADseq to generate SNP markers in a meiotic gynogenetic family of European seabass (Dicentrarchus labrax): (i) to verify the lack of paternal contribution in a meiotic gynogenetic family; (ii) to generate a gene-centromere map from this family; (iii) to identify telomeric markers that could distinguish mitotic gynogenetics from meiotic gynogenetics, which sometimes arise spontaneously in mitotic gynogenetic families. <br> Results: <br> From a single meiotic gynogenetic family consisting of 79 progeny, 42 million sequencing reads (Illumina, trimmed to 148 bases) resolved 6866 unique RAD-tags. The 340 male-informative SNP markers that were identified confirmed the lack of paternal contribution. A gene-centromere map was constructed based on 804 female-informative SNPs in 24 linkage groups (2n = 48) with a total length of 1251.02 cM (initial LG assignment was based on the seabass genome assembly, dicLab v1). Chromosome arm structure could be clearly discerned from the pattern of heterozygosity in each linkage group in 18 out of 24 LGs: the other six showed anomalies that appeared to be related to issues in the genome assembly. <br> Conclusion: <br> Genome-wide screening enabled substantive verification of the production of the gynogenetic family used in this study. The large number of telomeric and subtelomeric markers with high heterozygosity values in the meiotic gynogenetic family indicate that such markers could be used to clearly distinguish between meiotic and mitotic gynogenetics.en_US
dc.descriptionSource at: <a href=https://doi.org/10.1186/s12864-017-3826-z> https://doi.org/10.1186/s12864-017-3826-z </a>en_US
dc.identifier.citationOral, M., Colléter, J., Bekaert, M., Taggart, J. B., Palaiokostas, C., McAndrew, B. J., Vandeputte, M. … Penman, D. J. (2017). Gene-centromere mapping in meiotic gynogenetic European seabass. BMC Genomics, 18(449). https://doi.org/10.1186/s12864-017-3826-zen_US
dc.identifier.cristinIDFRIDAID 1474864
dc.identifier.doi10.1186/s12864-017-3826-z
dc.identifier.issn1471-2164
dc.identifier.urihttps://hdl.handle.net/10037/12166
dc.language.isoengen_US
dc.relation.journalBMC Genomics
dc.relation.urihttp://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-017-3826-z
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474en_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474en_US
dc.titleGene-centromere mapping in meiotic gynogenetic European seabassen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


File(s) in this item

Thumbnail

This item appears in the following collection(s)

Show simple item record