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dc.contributor.authorPerander, Maria
dc.contributor.authorAl-Mahdi, Rania
dc.contributor.authorJensen, Thomas Clemens
dc.contributor.authorNunn, Jennifer Ann Lillebo
dc.contributor.authorKildalsen, Hanne
dc.contributor.authorJohansen, Bjarne Herold
dc.contributor.authorGabrielsen, Mads
dc.contributor.authorKeyse, Stephen M
dc.contributor.authorSeternes, Ole Morten
dc.date.accessioned2018-03-05T12:39:52Z
dc.date.available2018-03-05T12:39:52Z
dc.date.issued2017-03-02
dc.description.abstractThe atypical MAP kinases ERK3 and ERK4 are activated by phosphorylation of a serine residue lying within the activation loop signature sequence S-E-G. However, the regulation of ERK3 and ERK4 phosphorylation and activity is poorly understood. Here we report that the inducible nuclear dual-specificity MAP kinase phosphatase (MKP) DUSP2, a known regulator of the ERK and p38 MAPKs, is unique amongst the MKP family in being able to bind to both ERK3 and ERK4. This interaction is mediated by a conserved common docking (CD) domain within the carboxyl-terminal domains of ERK3 and ERK4 and the conserved kinase interaction motif (KIM) located within the non-catalytic amino terminus of DUSP2. This interaction is direct and results in the dephosphorylation of ERK3 and ERK4 and the stabilization of DUSP2. In the case of ERK4 its ability to stabilize DUSP2 requires its kinase activity. Finally, we demonstrate that expression of DUSP2 inhibits ERK3 and ERK4-mediated activation of its downstream substrate MK5. We conclude that the activity of DUSP2 is not restricted to the classical MAPK pathways and that DUSP2 can also regulate the atypical ERK3/4-MK5 signalling pathway in mammalian cells.en_US
dc.descriptionSource at <a href=https://doi.org/10.1038/srep43471> https://doi.org/10.1038/srep43471 </a>.en_US
dc.identifier.citationPerander, M., Al-Mahdi, R., Jensen, T.C., Nunn, J.A.L., Kildalsen, H., Johansen, B.H. ... Seternes, O.M. (2017) Regulation of atypical MAP kinases ERK3 and ERK4 by the phosphatase DUSP2. Scientific Reports. 7:43471.en_US
dc.identifier.cristinIDFRIDAID 1513357
dc.identifier.doi10.1038/srep43471
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/10037/12248
dc.language.isoengen_US
dc.publisherNature Publishing Groupen_US
dc.relation.journalScientific Reports
dc.relation.projectIDThe National Programme for Research in Functional Genomics in Norway (FUGE)en_US
dc.relation.projectIDThe Norwegian Cancer Societyen_US
dc.relation.projectIDThe Blix Family Foundationen_US
dc.relation.projectIDNorthern Norway Regional Health Authority/Helse Nord RHFen_US
dc.relation.projectIDThe Aakre foundation (to O.M.S)en_US
dc.relation.projectIDCancer Research UKen_US
dc.rights.accessRightsopenAccessen_US
dc.subjectKinasesen_US
dc.subjectMedical researchen_US
dc.subjectVDP::Medisinske Fag: 700en_US
dc.subjectVDP::Medical disciplines: 700en_US
dc.titleRegulation of atypical MAP kinases ERK3 and ERK4 by the phosphatase DUSP2en_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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