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dc.contributor.authorRad, Amir
dc.contributor.authorSørbye, Sveinung Wergeland
dc.contributor.authorDreyer, Greta
dc.contributor.authorHovland, Siri
dc.contributor.authorFalang, Bente Marie
dc.contributor.authorLouw, Melanie
dc.contributor.authorSkjeldestad, Finn Egil
dc.date.accessioned2018-03-16T07:21:15Z
dc.date.available2018-03-16T07:21:15Z
dc.date.issued2017-11-01
dc.description.abstract<p>Accurate identification of human papillomavirus (HPV)-types in cervical cancer tissue may be important for tailoring tests for primary screening and types to be included in a vaccine. The aim of this study was to compare test-performance of a 45-type HPV deoxyribonucleic acid (DNA)-test with a 9-type HPV messenger ribonucleic acid (mRNA)-test in cervical cancer tissues. <p>In a case-series design 188 women with diagnosed cervical cancer during the period January 2008to July 1, 2011 at the Gynaecological Oncology Unit, University of Pretoria, South Africa were recruited to the study. After cases with negative internal controls for DNA/mRNA detection (n=18) and unconfirmed histology (n=3) of cervical cancer were excluded, 167 women remained eligible for analysis. We compared 45 DNA-types detected through general primer (GP)5+/6+ polymerase chain reaction (PCR) and reverse line blot (RLB) genotyping with a modified version of the mRNA test PreTect HPV-Proofer detecting 9 genotypes (16, 18, 31, 33, 35, 45, 51, 52, 58). <p>Histological types were 92.2% squamous cell carcinoma, 4.8% adenocarcinoma, and 3.0% adenosquamous carcinoma. Overall, HPV was detected in 95.2% (159/167) of specimens. The DNA- and mRNA tests each rendered 153/167 (91.6%) HPV positive results. When restricting the analysis to the 9 high-risk HPV-types included in the mRNA test, 91.6% (153/167) and 88.0% (147/167) were positive by the mRNA- and DNA-tests (P=.28), respectively. After hierarchical categorization of 9 comparable types, we found concordance in 66 of 67 specimens for HPV16, 25 of 27 specimens for HPV18, 19 of 21 specimens for HPV45, and only in 33 of 45 for HPV31, 33, 35, 51, 52, 58. The positivity rate for the HPV types 16, 18, and 45 and the positivity rate for HPV 16, 18, 45, 33 and 35 by both tests was 66% to 68% and 80% to 83%, respectively. <p>Overall and when considering established high-risk types, the mRNA test has at least as high detection rate as the DNA test. The mRNA test can be an appropriate research tool to describe causative HPV-types in cervical cancer tissue for health care planning purposes.en_US
dc.identifier.citationRad, A., Sørbye, S.W., Dreyer, G., Hovland, S., Falang, B.M., Louw, M. & Skjeldestad, F.E. (2017). HPV types in cervical cancer tissue in South Africa: A head-to-head comparison by mRNA and DNA tests. Medicine, 96(47).en_US
dc.identifier.cristinIDFRIDAID 1523107
dc.identifier.doi10.1097/MD.0000000000008752
dc.identifier.issn0025-7974
dc.identifier.issn1536-5964
dc.identifier.urihttps://hdl.handle.net/10037/12353
dc.language.isoengen_US
dc.publisherLippincott, Williams & Wilkinsen_US
dc.relation.ispartofRad, A. (2023). HPV mRNA and HPV DNA Tests in Cervical Cancer Screening. (Doctoral thesis). <a href=https://hdl.handle.net/10037/31583>https://hdl.handle.net/10037/31583</a>
dc.relation.journalMedicine (Baltimore, Md.)
dc.relation.urihttp://journals.lww.com/md-journal/Fulltext/2017/11270/HPV_types_in_cervical_cancer_tissue_in_South.60.aspx
dc.rights.accessRightsopenAccessen_US
dc.subject.hrcsKreft: Evaluering av markører og teknologier
dc.subject.hrcsCancer : Evaluation of markers and technologies
dc.subjectVDP::Medisinske fag: 700::Helsefag: 800::Samfunnsmedisin, sosialmedisin: 801en_US
dc.subjectVDP::Midical sciences: 700::Health sciences: 800::Community medicine, social medicine: 801en_US
dc.subjectMolekylærmedisin / Molecular medicineen_US
dc.subjectcervical canceren_US
dc.subjectDNA diagnosticsen_US
dc.subjectHPV-type distributionen_US
dc.subjectmRNA diagnosticsen_US
dc.subjectprevalenceen_US
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Onkologi: 762en_US
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750::Oncology: 762en_US
dc.titleHPV types in cervical cancer tissue in South Africa: A head-to-head comparison by mRNA and DNA testsen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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