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dc.contributor.authorRokstad, Anne Mari
dc.contributor.authorBrekke, Ole Lars
dc.contributor.authorSteinkjer, Bjørg
dc.contributor.authorRyan, Liv
dc.contributor.authorKolláriková, Gabriela
dc.contributor.authorStrand, Berit Løkensgard
dc.contributor.authorSkjåk-Bræk, Gudmund
dc.contributor.authorLacík, Igor
dc.contributor.authorEspevik, Terje
dc.contributor.authorMollnes, Tom Eirik
dc.date.accessioned2018-03-19T07:36:44Z
dc.date.available2018-03-19T07:36:44Z
dc.date.issued2011
dc.description.abstractAlginate microbeads and microcapsules are presently under evaluation for future cell-based therapy. Defining their inflammatory properties with regard to humans is therefore essential. A lepirudine-based human whole blood model was used as an inflammation predictor by measuring complement and leukocyte stimulation. Alginate microbeads were complement-compatible since they did not activate complement as measured by the soluble terminal complement complex (sTCC), Bb or the anaphylatoxins C3a and C5a. In addition, alginate microbeads were free of surface adherent leukocytes. In contrast, microcapsules containing poly-L-lysine (PLL) induced elevated levels of sTCC, Bb, C3a and C5a, surface active C3 convertase and leukocyte adhesion. The soluble PLL induced elevated levels of sTCC and up-regulated leukocyte CD11b expression. PMCG microcapsules containing poly(methylene-co-guanidine) complexed with sodium alginate and cellulose sulfate triggered a fast sTCC response and C3 deposition. The PMCG microcapsules were still less activating than PLL-containing microcapsules as a function of time. The amounts of anaphylatoxins C3a and C5a were diminished by the PMCG microcapsules, whereas leukocyte adherence demonstrated surface activating properties. We propose the whole blood model as an important tool for measuring bioincompatibility of microcapsules and microbeads for future applications as well as determining the mechanisms leading to inflammatory reactions.en_US
dc.descriptionAccepted manuscript version. Published version available in <a href=http://dx.doi.org/10.1016/j.actbio.2011.03.011> Acta Biomaterialia. 2011, 7 (6), 2566-2578. http://dx.doi.org/10.1016/j.actbio.2011.03.011. </a>en_US
dc.identifier.citationRokstad, A. M., Brekke, O., Steinkjer, B., Ryan, L., Kolláriková, G., Strand, B. L. ... Mollnes, T. E. (2011). Alginate microbeads are complement compatible, in contrast to polycation containing microcapsules, as revealed in a human whole blood model. Acta Biomaterialia. 7(6):2566-2578en_US
dc.identifier.cristinIDFRIDAID 801295
dc.identifier.doi10.1016/j.actbio.2011.03.011
dc.identifier.issn1742-7061
dc.identifier.issn1878-7568
dc.identifier.urihttps://hdl.handle.net/10037/12367
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalActa Biomaterialia
dc.rights.accessRightsopenAccessen_US
dc.subjectAlginateen_US
dc.subjectMicrocapsuleen_US
dc.subjectMicrobeaden_US
dc.subjectComplementen_US
dc.subjectBiocompatibilityen_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk immunologi: 716en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical immunology: 716en_US
dc.titleAlginate microbeads are complement compatible, in contrast to polycation containing microcapsules, as revealed in a human whole blood modelen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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