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dc.contributor.authorHelland, Åslaug
dc.contributor.authorBrustugun, Odd Terje
dc.contributor.authorNakken, Sigve
dc.contributor.authorHalvorsen, Ann Rita
dc.contributor.authorDønnem, Tom
dc.contributor.authorBremnes, Roy M.
dc.contributor.authorBusund, Lill-Tove
dc.contributor.authorSun, Jinchang
dc.contributor.authorLorenz, Susanne
dc.contributor.authorSolberg, Steinar
dc.contributor.authorJørgensen, Lars Hilmar
dc.contributor.authorVodak, Daniel
dc.contributor.authorMyklebost, Ola
dc.contributor.authorHovig, Eivind
dc.contributor.authorMeza, Leonardo Zepeda
dc.date.accessioned2018-03-25T06:34:15Z
dc.date.available2018-03-25T06:34:15Z
dc.date.issued2017-04-07
dc.description.abstractLung cancer is the leading cause of cancer related death, and the past years’ improved insight into underlying molecular events has significantly improved outcome for specific subsets of patients. In particular, several new therapies that target protein kinases have been implemented, and many more are becoming available. We have investigated lung cancer specimens for somatic mutations in a targeted panel of 612 human genes, the majority being protein kinases. The somatic mutation profiles were correlated to profiles of immune cell infiltration as well as relapse‐free survival. Targeted deep sequencing was performed on 117 tumour/normal pairs using the SureSelect Human Kinome kit (Agilent Technologies), with capture probes targeting 3.2 Mb of the human genome, including exons and untranslated regions of all known kinases, kinase receptors and selected cancer‐related genes (612 genes in total). CD8 staining was determined using Ventana Benchmark. Survival analyses were performed using SPSS. The number of mutations per sample ranged from 0 to 50 (within the 612 genes tested), with a median of nine. The prognosis was worse for patients with more than the median number of mutations. A significant correlation was found between mutations in one of selected DNA‐repair genes and the total number of mutations in that tumour (p < 0.001). There was a significant inverse correlation between the number of infiltrating stromal CD8+ lymphocytes and the presence of EGFR mutations.en_US
dc.descriptionSource at <a href=https://doi.org/10.1002/ijc.30726> https://doi.org/10.1002/ijc.30726 </a>en_US
dc.identifier.citationHelland, Å., Brustugun, O. T., Nakken, S., Halvorsen, A. R., Dønnem, T., Bremnes, R. M. ... Meza, Z. L. (2017 )High number of kinome-mutations in non-small cell lung cancers associated with reduced immune response and poor relapse-free survival. International Journal of Cancer. 141(1):184-190en_US
dc.identifier.cristinIDFRIDAID 1536909
dc.identifier.doi10.1002/ijc.30726
dc.identifier.issn0020-7136
dc.identifier.issn1097-0215
dc.identifier.urihttps://hdl.handle.net/10037/12429
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.journalInternational Journal of Cancer
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Onkologi: 762en_US
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750::Oncology: 762en_US
dc.titleHigh number of kinome-mutations in non-small cell lung cancers associated with reduced immune response and poor relapse-free survivalen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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