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dc.contributor.authorPaunas, Theodora Ioana Flavia
dc.contributor.authorFinne, Kenneth
dc.contributor.authorLeh, Sabine
dc.contributor.authorMarti, Hans-Peter
dc.contributor.authorMollnes, Tom Eirik
dc.contributor.authorBerven, Frode
dc.contributor.authorVikse, Bjørn Egil
dc.date.accessioned2018-05-02T13:33:36Z
dc.date.available2018-05-02T13:33:36Z
dc.date.issued2017-08-14
dc.description.abstractBackground: The clinical course of IgA nephropathy (IgAN) is variable and complement activation may predict prognosis. The present study investigated whether glomerular abundance of complement proteins associates with progression to end-stage renal disease (ESRD) in patients for whom prognosis could not be predicted based on clinical variables. <br> <br> Methods: Based on data from the Norwegian Kidney Biopsy Registry and the Norwegian Renal Registry, three groups were included: IgAN patients with (n = 9) or without (n = 16) progression to ESRD during 10 years, and controls (n = 15) with a normal kidney biopsy. IgAN patients had eGFR > 45 ml/min/1.73 m2 and non-nephrotic proteinuria at time of biopsy. Using stored formalin-fixed paraffin embedded kidney biopsy tissue, about 100 glomerular cross sections were microdissected for each patient. Samples were analyzed by liquid chromatography–tandem mass spectrometry and relative abundances of complement proteins were compared between groups. <br> <br> Results: Proteomic analyses quantified 2018 proteins, of which 28 proteins belong to the complement system. As compared to IgAN patients without progressive disease, glomeruli from patients with progressive IgAN had significantly higher abundance of components of the classical and the terminal complement pathways, and inhibitory factors such as Factor H and factor H related proteins. Abundance of complement proteins classified progressors from non-progressors with an area under ROC curve of 0.91 (p = 0.001). Clinical and morphological data were similar between the two patient groups and could not predict progressive IgAN. <br> <br> Conclusions: In conclusion, higher glomerular abundance of complement proteins was associated with a progressive clinical course in IgAN and are candidate biomarkers to predict prognosis.en_US
dc.description.sponsorshipThe Western Norway Regional Health Authority and Haugesund Hospital.en_US
dc.descriptionSource at <a href=https://doi.org/10.1186/s12014-017-9165-x>https://doi.org/10.1186/s12014-017-9165-x</a>.en_US
dc.identifier.citationPaunas, T.I.F., Finne, K., Leh, S., Marti, H.P., Mollnes, T.E., Berven, F. & Vikse, B.E. (2017). Glomerular abundance of complement proteins characterized by proteomic analysis of laser-captured microdissected glomeruli associates with progressive disease in IgA nephropathy. Clinical Proteomics,14(30). https://doi.org/10.1186/s12014-017-9165-xen_US
dc.identifier.cristinIDFRIDAID 1543414
dc.identifier.doi10.1186/s12014-017-9165-x
dc.identifier.issn1542-6416
dc.identifier.issn1559-0275
dc.identifier.urihttps://hdl.handle.net/10037/12678
dc.language.isoengen_US
dc.publisherBioMed Centralen_US
dc.publisheren_US
dc.relation.journalClinical Proteomics
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Nefrologi, urologi: 772en_US
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750::Nephrology, urology: 772en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Generell patologi, patologisk anatomi: 719en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::General pathology, anatomical pathology: 719en_US
dc.subjectIgA nephropathyen_US
dc.subjectComplementen_US
dc.subjectESRDen_US
dc.subjectFormalin-fixed paraffinen_US
dc.subjectKidney biopsy tissueen_US
dc.subjectLiquid chromatographyen_US
dc.subjectTandem mass spectrometryen_US
dc.subjectProteomic analysesen_US
dc.titleGlomerular abundance of complement proteins characterized by proteomic analysis of laser-captured microdissected glomeruli associates with progressive disease in IgA nephropathyen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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