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dc.contributor.authorHennø, Linda Torrissen
dc.contributor.authorStorjord, Elin
dc.contributor.authorChristiansen, Dorte
dc.contributor.authorBergseth, Grete
dc.contributor.authorLudviksen, Judith K
dc.contributor.authorFure, Hilde
dc.contributor.authorBarene, Svein
dc.contributor.authorNielsen, Erik Waage
dc.contributor.authorMollnes, Tom Eirik
dc.contributor.authorBrekke, Ole Lars
dc.date.accessioned2018-08-16T13:22:37Z
dc.date.available2018-08-16T13:22:37Z
dc.date.issued2017-06-06
dc.description.abstractCytokines are potentially useful biomarkers of sepsis and other inflammatory conditions. Many cytokines can be released by leukocytes and platelets after sampling. The sampling and processing techniques are consequently critically important to measure the <i>in vivo</i> levels. We therefore examined the effects of four different anticoagulants, EDTA, citrate, lepirudin, heparin compared to serum, on the levels of 27 different cytokines. The effects of storage temperature, freezing and thawing on the plasma cytokines were examined. Cytokines were analysed using a multiplex immunoassay. The cytokine levels in serum were significantly higher compared with plasma, consistent with release of cytokines <i>in vitro</i> during coagulation. In general, the lowest values for all cytokines were found in EDTA samples, stored on crushed ice, centrifuged within 4 h and thereafter stored at −80 °C. MCP-1 and MIP-1β levels were highest in heparin plasma and storage of blood for up to 4 h at room temperature significantly increased the interleukin (IL)-2, IL-6, IL-8, IFN-γ and GM-CSF levels in EDTA plasma, indicating post-sampling release. In contrast, the IP-10 levels were unaffected by sample storage at both temperatures. Our results indicate that the cytokines were more stable in plasma than in whole blood after sampling. Thus, cytokines should be analysed in EDTA plasma samples stored on ice and centrifuged within 4 h. Based on these data, the reference ranges of 27 cytokines in EDTA plasma in 162 healthy human donors were calculated.en_US
dc.description.sponsorshipHelse Nord RHFen_US
dc.identifier.citationHennø, L.T., Storjord, E., Christiansen, D., Bergseth, G., Ludviksen, J.K., Fure, H., ... Brekke, O. (2017). Effect of the anticoagulant, storage time and temperature of blood samples on the concentrations of 27 multiplex assayed cytokines – Consequences for defining reference values in healthy humans. Cytokine, 97, 86-95. https://doi.org/10.1016/j.cyto.2017.05.014en_US
dc.identifier.cristinIDFRIDAID 1472266
dc.identifier.doi10.1016/j.cyto.2017.05.014
dc.identifier.issn1043-4666
dc.identifier.issn1096-0023
dc.identifier.urihttps://hdl.handle.net/10037/13444
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.ispartofStorjord, E. (2021). Acute intermittent porphyria. Inflammation, diet and biomarkers in acute intermittent porphyria. (Doctoral thesis). <a href=https://hdl.handle.net/10037/20120>https://hdl.handle.net/10037/20120</a>
dc.relation.journalCytokine
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/SFF/223255/Norway/Centre of Molecular Inflammation Research/CEMIR/en_US
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750en_US
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750en_US
dc.subjectEDTAen_US
dc.subjectethylenediamine tetraacetic aciden_US
dc.subjectILen_US
dc.subjectinterleukinen_US
dc.subjectRTen_US
dc.subjectroom temperatureen_US
dc.subjectAnticoagulanten_US
dc.subjectComplementen_US
dc.subjectCytokinesen_US
dc.subjectLepirudinen_US
dc.subjectPlasmaen_US
dc.titleEffect of the anticoagulant, storage time and temperature of blood samples on the concentrations of 27 multiplex assayed cytokines – Consequences for defining reference values in healthy humansen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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